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Western blot - Corticotropin Releasing Factor Receptor 2 antibody (ab75168)
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Product Name
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Corticotropin Releasing Factor Receptor 2 antibody
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See all Corticotropin Releasing Factor Receptor 2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Corticotropin Releasing Factor Receptor 2
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Immunogen
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synthetic peptide derived from internal region of human Corticotropin Releasing Factor Receptor 2
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab75168 detects endogenous levels of total CRFR2 protein
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Predicted molecular weight: 48 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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extracts from K562 cells, LOVO cells, 3T3 cells and RAW264.7 cells
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Cellular localization
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Multi-pass membrane protein.
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Research areas
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Neuroscience >> Endocrine system >> hypothalamic pituitary adrenal axis
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
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Relevance
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Corticotropin Releasing Factor Receptor 2 (CRHR2) expression has been reported in various regions of the brain, as well as in placenta, umbilical vein, heart, epididymis, gastrointestinal tract, adrenal, and skeletal muscle. It shows high-affinity CRF binding and also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. CRCH2 alpha is the dominant isoform and is expressed widely. CRCH2 beta is expressed in the hippocampus, septum, amygdala, heart, and skeletal muscle. CRHR2 gamma is brain-specific. ESTs have been isolated from brain libraries.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Corticotropin Releasing Factor Receptor 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Corticotropin Releasing Factor Receptor 2 antibody images, references, products related to ab75168 and other tools.
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Corticotropin Releasing Factor Receptor 2 antibody images:
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 Western blot - Corticotropin Releasing Factor Receptor 2 antibody (ab75168)
All lanes : Corticotropin Releasing Factor Receptor 2 antibody (ab75168) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from LOVO
cells
Lane 3 : extracts from 3T3 cells
Lane 4 : extracts from RAW264.7 cells
Lane 5 : extracts from 3T3 cells with immunising peptide at 10ug
Lysates/proteins at 30 µg per lane.
Predicted band size : 48 kDa
Observed band size : 48 kDa
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Corticotropin Releasing Factor Receptor 2
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Corticotropin Releasing Factor Receptor 2 antibody - more information
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