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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Overview
Product name
Anti-Corticotropin Releasing Factor Receptor 2 antibody
See all Corticotropin Releasing Factor Receptor 2 products (2) ...
Description
Rabbit polyclonal to Corticotropin Releasing Factor Receptor 2
Specificity
ab75168 detects endogenous levels of total CRFR2 protein
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
synthetic peptide derived from internal region of human Corticotropin Releasing Factor Receptor 2
Positive control
extracts from K562 cells, LOVO cells, 3T3 cells and RAW264.7 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 48 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Target
Function
This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Sequence similarities
Belongs to the G-protein coupled receptor 2 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ13324
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Corticotropin releasing hormone receptor 2 antibody
- Corticotropin-releasing factor receptor 2 antibody
- Corticotropin-releasing hormone receptor 2 antibody
see all
Database links
- Entrez Gene: 1395 Human
- Entrez Gene: 12922 Mouse
- Entrez Gene: 64680 Rat
- Omim: 602034 Human
- SwissProt: Q13324 Human
- SwissProt: Q60748 Mouse
- SwissProt: P47866 Rat
- Unigene: 546246 Human
see all
Anti-Corticotropin Releasing Factor Receptor 2 antibody images:
Western blot - Corticotropin Releasing Factor Receptor 2 antibody (ab75168)
All lanes : Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab75168) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from LOVO
cells
Lane 3 : extracts from 3T3 cells
Lane 4 : extracts from RAW264.7 cells
Lane 5 : extracts from 3T3 cells with immunising peptide at 10ug
Lysates/proteins at 30 µg per lane.
Predicted band size : 48 kDa
Observed band size : 48 kDa
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Corticotropin Releasing Factor Receptor 2 antibody(ab75168)
IHC image of ab75168 staining in human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab75168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab75168)
ab75168 has not yet been referenced specifically in any publications.
Publishing research using ab75168? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"