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Anti-Corticotropin Releasing Factor antibody [4H9] (ab35748)

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Overview

Product name

Anti-Corticotropin Releasing Factor antibody [4H9]
See all Corticotropin Releasing Factor products (8) ...

Description

Mouse monoclonal [4H9] to Corticotropin Releasing Factor

Specificity

Reacts with CRF. Does not react with bovine serum albumin, insulin, ovalbumin or epidermal growth factor.

Tested applications

ELISA, IHC-P, IHC-Fr, Inhibition Assaymore details

Cross reactivity

Reacts with

Human

Predicted to work with

Rat

Immunogen

Full length protein (Human)

Epitope

The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant of ab35748. Lysine modification by CA blocks binding completely.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

4H9

Isotype

IgG1

Light chain type

kappa

Research areas

Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Signal Transduction >> Growth Factors/Hormones >> Hormones
Neuroscience >> Neurotransmitter >> Neuropeptides >> Hormones

  • Immunohistochemistry (Frozen sections) - Corticotropin Releasing Factor antibody [4H9] (ab35748)Immunohistochemistry (Frozen sections) - Corticotropin Releasing Factor antibody [4H9] (ab35748) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab35748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

  • ShowHide

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

  • 1 Image

    IHC-Fr

    Four stars (2 Abreviews) IHC-Fr: 1/100 - 1/7000.

    IHC-Fr: 1/100 - 1/7000.

  • ShowHide

    Inhibition Assay

     Inhib: Use at an assay d...Read more →

    Inhib: Use at an assay dependent dilution. (Human serum, containing CRF-binding protein, inhibits the binding of CRF to this antibody.)

Target

Relevance

CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).

Cellular localization

Secreted

Alternative names

  • Corticoliberin antibody
  • Corticoliberin precursor antibody
  • Corticotropin releasing factor antibody
  • Corticotropin releasing hormone antibody
  • Corticotropin releasing hormone deficiency included antibody
  • crf antibody
  • crh antibody
  • crh deficiency included antibody
see all

Database links

Anti-Corticotropin Releasing Factor antibody [4H9] images:

  Immunohistochemistry (Frozen sections) - Corticotropin Releasing Factor antibody [4H9] (ab35748)

Immunohistochemistry (Frozen sections) - Corticotropin Releasing Factor antibody [4H9] (ab35748)

ab35748 staining Corticotrophin Releasing Factor in Rat brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in methanol prior to blocking with PBS with 1.5% goat serum for 20 minutes at 22°C. The primary antibody was diluted 1/100 in the blocking buffer and incubated with the sample for 10 hours at 4°C. A Dylight® 594-conjugated polyclonal antibody was used as the secondary antibody, diluted 1/80.

Image courtesy of an Abreview submitted by Nitsan Kozlovsky.

See Abreview

References for Anti-Corticotropin Releasing Factor antibody [4H9] (ab35748)

This product has been referenced in:

  • Kravchenko IV & Furalev VA Monoclonal antibodies directed against two different corticotropin-releasing factor determinants. Hybridoma 13:59-64 (1994).Read more (PubMed: 7515375) »

See 1 publication for this product

Publishing research using ab35748? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"