Corticotropin Releasing Factor antibody [4H9] (ab35748)

Product Name 

Corticotropin Releasing Factor antibody [4H9]

Product type 

Primary antibodies

Description 

Mouse monoclonal [4H9] to Corticotropin Releasing Factor

Immunogen 

Full length protein (Human)

Reacts with 

Hu

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Specificity 

Reacts with CRF. Does not react with bovine serum albumin, insulin, ovalbumin or epidermal growth factor.

Epitope 

The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant of ab35748. Lysine modification by CA blocks binding completely.

Tested applications 
(see key)

ELISA, IHC-Fr, IHC-P, Inhib

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Enzyme digestion with 0.1% trypsin may also be necessary, ABC is recommended for detection).
IHC-Fr: 1/100 - 1/7000.
Inhib: Use at an assay dependent dilution. Human serum, containing CRF-binding protein, inhibits the binding of CRF to this antibody.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Neuroscience >> Endocrine system >> hypothalamic pituitary adrenal axis
Signal Transduction >> Growth Factors/Hormones >> Hormones
Neuroscience >> Neurotransmitter >> Neuropeptides >> Hormones

Relevance 

CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

4H9

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for Corticotropin Releasing Factor antibody [4H9] (ab35748)

  This clone has been used in:

Kravchenko IV & Furalev VA Monoclonal antibodies directed against two different corticotropin-releasing factor determinants. Hybridoma 13:59-64 (1994). PubMed: 7515375

If you publish research using ab35748 please let us know so that we can cite the reference on this datasheet.

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