Products:Neuroscience >> Neurotransmitter >> Neuropeptides >> Hormones
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ab8901 has been referenced in 4 publications.
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Radioimmunoassay using ab8901
- Day 1: Add Recombinant Protein A/G (10ug/ml) to a 96 wells plate; parafilm wrap; incubate overnight at 4ºC.
- Day 2: Aspirate and wash the plate to remove loosely bound Protein A/G; add antibody solution (dilution 1:50000); parafilm wrap; incubate 24h at 4ºC.
- Day 3: Aspirate and wash the plate to remove loosely bound antibody; add CRF standard (0, 0.03, 0.1, 0.3, 1, 3, 10, 30 ng/ml); parafilm wrap; incubate 24h at 4ºC.
- Day 4: DO NOT ASPIRATE; add 125I-CRF; incubate 24h at 4ºC.
- Day 5: Aspirate and wash the plate; break the plate and collect the wells in scintillation tubes; add 2ml of scintillation liquid; count in beta counter (open the 3H window to at least 30-40 keV), at least 2min/tube.
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