Anti-Corticotropin Releasing Factor antibody (ab8901)

Oh ok, this is great then. I am attaching the review so that you can process it manually, since there is no online form.

ANSWER:

Thank you for your reply. I have published your Abreview on our online datasheet and awarded Abpoints to your user account. You can view and redeem your Abpoints by logging in to your account on our website.

Additionally, since you have submitted an image of an application that did not previously have an image, the discount code *** will allow you to receive a free primary antibody on your next purchase from us before September 2nd, 2012. This code can be used by anyone in your institution, and the order can be placed by phone, fax, email, or online.

I hope this helps, please let me know if you need any other information or assistance.

What size band does ab8901 produce for Western blotting?
Thanks!

ANSWER:

Thank you for contacting Abcam regarding ab8901.


The predicted molecular weight of this protein is 21kDa. In WB you should detect a band at approximately this size.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

We are interested in the discount and will be placing the order for the 100ul vial later today. Look forward to that discount.

ANSWER:

I am very pleased to hear you would like to use ab8901 in RIA and send us your data and an image of your results.

This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview with the image for RIA and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please read the following:

Typically, researchers can submit a product review via an online submission form. At this time we do not have an Abreview form available for submitting reviews on this product. As an alternative, please email your review of ab8901 to me.

In the review, please include:
1) A brief description of your experimental procedures
2) An attached JPEG or PNG formatted image illustrating your data
2) Your feelings on the results
3) If you would like your name to be shown with your review or if you prefer to be anonymous

Regarding your image, please note any descriptive details that will help other researchers interpret your results.

You will be awarded the same number of points as if you had submitted the review online using a standard Abreview form. You will receive 120 points for text with an additional 100 points for including an image. These points can be used for discounts on future purchases or Amazon gift cards.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

We will certainly use these papers for protocol, but neither one specifies the dilution used. The datasheet produced by Abcam indicates 1:600,000 as the recommended dilution - how was this determined?

ANSWER:

Thank you for your reply.

I do apologize as I have been unable to obtain any more specific information regarding the dilution of this antibody to be used in RIA.

Due to this lack of specific details, I would like to offer you a testing discount, whereby you would purchase the antibody and test it in RIA and then submit your protocol and data to me which would be published on our website as an Abreview. Once that review has been received, you would be eligible to receive a free primary antibody on a future order.


Plesae let me know if this would be of interest to you and I will issue you a discount code. I do apologize for any inconvenience caused. I look forward to your reply so that I may assist you further.

Can you confirm the correct dilution to use this antibody in RIA? What is the recommended protocol?

ANSWER:

Thank you for contacting Abcam regarding ab8901.


I have confirmed with the laboratory that RIA was not tested in house, but based on the following references:

1. Widmaier EP, Lim AT, Vale W. (1989) Secretion of corticotropin-releasing factor from cultured rat hypothalamic cells: effects of catecholamines. Endocrinol 124:583-590.

2. Vale W, Vaughan J, Yamamoto G, Bruhn T, Douglas C, Dalton D, Rivier C, Rivier J. (1983) Assay of corticotopin releasing factor. Meth Enzymol 103:565-577.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.