Recombinant Anti-Cortisol Binding Globulin antibody [EPR4615] (ab110648)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4615] to Cortisol Binding Globulin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cortisol Binding Globulin antibody [EPR4615]
See all Cortisol Binding Globulin primary antibodies -
Description
Rabbit monoclonal [EPR4615] to Cortisol Binding Globulin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal artery, plasma and placenta lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4615 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. -
Tissue specificity
Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells. -
Involvement in disease
Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency) [MIM:611489]. CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsN-glycosylated; binds 5 oligosaccharide chains.
Glycosylation in position Asn-260 is needed for steroid binding. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 866 Human
- Omim: 122500 Human
- SwissProt: P08185 Human
- Unigene: 532635 Human
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Alternative names
- CBG antibody
- CBG_HUMAN antibody
- corticosteroid binding globulin antibody
see all
Images
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All lanes : Anti-Cortisol Binding Globulin antibody [EPR4615] (ab110648) at 1/1000 dilution
Lane 1 : Human fetal artery lysate
Lane 2 : Human plasma lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab110648 has been referenced in 1 publication.
- Wang C et al. Screening and identification of endometrial proteins as novel potential biomarkers for repeated implantation failure. PeerJ 9:e11009 (2021). PubMed: 33763303