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Overview

  • Product nameCortisol Human ELISA KitSee all Cortisol kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 5 ng/ml
  • Range
    5 ng/ml - 500 ng/ml
  • Recovery

    = 102.2 %

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108665, Cortisol Human ELISA Kit is a competitive immunoenzymatic colorimetric method intended for the quantitative determination of Cortisol in human serum and plasma.

    Cortisol is a steroid hormone released from the adrenal cortex in response to a hormone called ACTH (produced by the pituitary

    gland), it is involved in the response to stress; it increases blood pressure, blood sugar levels, may cause infertility in women, and

    suppresses the immune system.

    Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function,

    glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an

    unbound (free) form.

    Cortisol is bound with high affinity in plasma from corticosteroid-binding globulin (CBG, transcotin) and from albumin. Only free

    cortisol is available to most receptors.

  • Tested applicationsCompetitive ELISA more details

Properties

Applications

Our Abpromise guarantee covers the use of ab108665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Competitive ELISA cELISA

Protocols

References for Cortisol Human ELISA Kit (ab108665)

ab108665 has not yet been referenced specifically in any publications.

Publishing research using ab108665 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab108665 Submit a Question for ab108665

Displaying 1 - 5 of 5 results for Abreviews and Q&A

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Votes: Highest First

We have bought two abcam ELISA kits to dose testosterone (ab108666) and
cortisol (ab108665) from human blood samples.
Unfortunately, blood samples were frozen without separating blood
products (no centrifugation before freezing). As a resul...

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I confirmed with the lab and unfortunately do not have good news:
The heavily hemolyzed samples would have an great influence on the results. We do not know of any protocol trick to circumvent this and therefore recommend to collect new samples....

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I am using this kit for mouse samples, and the amount of sample is small. can i use EDTA or something else to prevent clotting, so I can maximaze the amount of sample i get?

Thank you for contacting us.

The Cortisol ELISA kit is validated for use on Human serum and plasma samples. We have not validated the use of Mouse samples in this kit, however, plasma samples have been validated. As such, it should be ok to colle...

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Avez-vous également des kits ELISA pour doser de l'ACTH plasmatique et du CRH spinal (échantillon de liquide céphalo-rachidien) chez le macaque s'il vous plait?

Malheureusement non, nous n'avons pas de kit pour le dosage de l'ACTH ou du CRH spinal.

Désolé de ne pas pouvoir vous aider davantage. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.


Je me permets de vous contacter sur les conseils d'Evelyne Doudnikoff.
Je suis à la recherche d'un kit ELISA pour doser du cortisol plasmatique chez le macaque.
Vendez-vous ce produit s'il vous plait?
Si oui, pourrais-je avoir le p...

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Merci de nous avoir contactés.

Nous avons à notre catalogue le kit http://www.abcam.com/ab108665, Cortisol Human (competitive)ELISA Kit.


Ce kit a été testé chez l'humain uniquement, il fonctionne ...

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Does this Cortisol ELISA Kit recognize total or free Cortisol?

Thank you for contacting us.  The Cortisol ELISA Kit ab108665 detects total Cortisol.  I hope this helps, please let me know if you need any additional information. 

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"