Overview
- Product nameCortisol Human ELISA KitSee all Cortisol kits ...
- Tests1 x 96 test
- Sample typeUrine
- Assay typeCompetitive
- Sensitivity2 ng/ml
- Range10 ng/ml - 500 ng/ml
- Recovery
105 %
- Species reactivityReacts with: Human
- Product overview
ab108674 Cortisol Human ELISA Kit uses a competitive immunoenzymatic colorimetric method for quantitative determination of Cortisol in urine.
Cortisol is a steroid hormone released from the adrenal cortex in response to an hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress; it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. Cortisol is bound, in plasma, by corticosteroid-binding globulin (CBG, transcotin), with high affinity, and by albumin. Only free cortisol is available to most receptors. These normal endogenous functions are the basis for the physiological consequences of chronic stress - prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs. The free cortisol fraction represents the metabolically active cortisol. In normal conditions, less than 1% of cortisol becomes excreted in urine.
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Anti-Cortisol IgG Coated Wells 12 x 8 tests Cortisol Standards 5 x 1ml Cortisol-HRP Conjugate 1 x 0.4ml Cover foils 2 units High control 1 x 1ml Incubation Buffer 1 x 100ml Low control 1 x 1ml Stop Solution 1 x 12ml Strip holder 1 unit TMB Substrate Solution 1 x 12ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab108674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Protocols
References for Cortisol Human ELISA Kit (ab108674)
ab108674 has not yet been referenced specifically in any publications.
