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Immunocytochemistry/ Immunofluorescence - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)
![Immunocytochemistry/ Immunofluorescence - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)](\ps\datasheet\images\9\ab9891\Coxsackie-Adenovirus-Receptor-Primary-antibodies-ab9891-1.jpg)
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Product Name
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Coxsackie Adenovirus Receptor antibody [E1-1]
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See all Coxsackie Adenovirus Receptor antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [E1-1] to Coxsackie Adenovirus Receptor
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Immunogen
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Recombinant fusion protein consisting of the extracellular human CAR-domain fused to the human IgG1-Fc domain.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Specificity
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Human CAR
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 50µl of antibody and a 100 µl suspension of maximally 1 million cells.
IHC-Fr: 1/10.
WB: 1/160. Use under non reducing conditions.
ICC/IF: 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Suggested positive control cell lines include: human embryonal cell lines 293 and 911, cervical carcinoma cell line, HeLa, lung carcinoma cell line A549, Burkitt's lymphoma Raji and Ramos.
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Cellular localization
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Cell Membrane and Secreted
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Microbiology >> Interspecies Interaction >> Host Virus Interaction
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Relevance
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Coxsackie and adenovirus receptor (CAR), also known as CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity. It is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.It is proposed to function as a homophilic cell adhesion molecule and recruits MPDZ to intercellular contact sites. CAR is probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
5 isoforms have been identified. Isoforms 1 & 2 are localized to the cell membrane whereas isoforms 3, 4, and 5 are secreted.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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E1-1
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 10% Fetal calf serum, IDMEM, 20mM HEPES Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Coxsackie Adenovirus Receptor antibody [E1-1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Coxsackie Adenovirus Receptor antibody [E1-1] images, references, products related to ab9891 and other tools.
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Coxsackie Adenovirus Receptor antibody [E1-1] images:
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 Immunocytochemistry/ Immunofluorescence - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)
ICC/IF image of ab9891 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9891, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)
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This product has been used in: (two most recent references)
Fernandes MS et al. Growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 ligand transgene. Clin Cancer Res 15:4847-56 (2009). Flow Cyt. PubMed: 19622582
Sonabend AM et al. Mesenchymal stem cells effectively deliver an oncolytic adenovirus to intracranial glioma. Stem Cells 26:831-41 (2008). Flow Cyt; Human. PubMed: 18192232
See all 10 publication references for this product.
If you publish research using ab9891 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Coxsackie Adenovirus Receptor
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Coxsackie Adenovirus Receptor antibody [E1-1] - more information
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Customer reviews (feedback) regarding Coxsackie Adenovirus Receptor antibody [E1-1] |
Customer FAQs regarding Coxsackie Adenovirus Receptor antibody [E1-1] |
Protocols for Coxsackie Adenovirus Receptor antibody [E1-1] |
Price and availability of products related to Coxsackie Adenovirus Receptor antibody [E1-1] |
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Coxsackie Adenovirus Receptor antibody [E1-1] - related products:
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Coxsackie Adenovirus Receptor antibody [E1-1] - other tools:
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Contact Abcam with a Technical Enquiry about Coxsackie Adenovirus Receptor antibody [E1-1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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