![Immunocytochemistry/ Immunofluorescence - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891) image](#Coxsackie-Adenovirus-Receptor-Primary-antibodies-ab9891-1.jpg)
![Western blot - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891) image](#Coxsackie-Adenovirus-Receptor-Primary-antibodies-ab9891-6.jpg)
 image](#Coxsackie-Adenovirus-Receptor-Primary-antibodies-ab9891-7.jpg)
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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-Coxsackie Adenovirus Receptor antibody [E1-1]
See all Coxsackie Adenovirus Receptor products (6) ...
Description
Mouse monoclonal [E1-1] to Coxsackie Adenovirus Receptor
Specificity
Human CAR
Tested applications
Flow Cyt, ICC/IF, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Recombinant fusion protein consisting of the extracellular human CAR-domain fused to the human IgG1-Fc domain.
Positive control
Suggested positive control cell lines include: human embryonal cell lines 293 and 911, cervical carcinoma cell line, HeLa, lung carcinoma cell line A549, Burkitt's lymphoma Raji and Ramos.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.476% HEPES, 10% Foetal calf serum, IDMEM
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
E1-1
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
ICC/IF: 1/1000
IHC-Fr
IHC-Fr: 1/10IHC-Fr: 1/10
WB: 1/160 Use under non reducing condition. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Target
Function
Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
Tissue specificity
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Domain
The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
Post-translational
modifications
N-glycosylated.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
Cellular localization
Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
Target information above from: UniProt accessionP78310
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
- 46 kD coxsackievirus and adenovirus receptor protein antibody
- CAR antibody
see all
Database links
- Entrez Gene: 1525 Human
- Omim: 602621 Human
- SwissProt: P78310 Human
- Unigene: 634837 Human
Anti-Coxsackie Adenovirus Receptor antibody [E1-1] images:
Immunocytochemistry/ Immunofluorescence - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)
![Immunocytochemistry/ Immunofluorescence - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)](/ps/datasheet/images/9/ab9891/Coxsackie-Adenovirus-Receptor-Primary-antibodies-ab9891-1.jpg)
ICC/IF image of ab9891 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9891, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)
![Western blot - Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)](/ps/datasheet/images/9/ab9891/Coxsackie-Adenovirus-Receptor-Primary-antibodies-ab9891-6.jpg)
Anti-Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891) at 1/500 dilution + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Flow Cytometry-Anti-Coxsackie Adenovirus Receptor antibody [E1-1](ab9891)
](/ps/datasheet/images/9/ab9891/Coxsackie-Adenovirus-Receptor-Primary-antibodies-ab9891-7.jpg)
Overlay histogram showing HeLa cells stained with ab9891 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9891, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-Coxsackie Adenovirus Receptor antibody [E1-1] (ab9891)
This product has been referenced in:
- Jornot Let al. N-acetylcysteine augments adenovirus-mediated gene expression in human endothelial cells by enhancing transgene transcription and virus entry. J Gene Med 4:54-65 (2002).Read more (PubMed: 11828388) »
- Kim Jet al. Active targeting of RGD-conjugated bioreducible polymer for delivery of oncolytic adenovirus expressing shRNA against IL-8 mRNA. Biomaterials 32:5158-66 (2011). Flow Cyt; Human.Read more (PubMed: 21531456) »
See all 12 publications for this product
Publishing research using ab9891? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"