Anti-Creatine kinase B type antibody (ab38211)
Overview
- Product nameAnti-Creatine kinase B type antibodySee all Creatine kinase B type primary antibodies ...
- DescriptionRabbit polyclonal to Creatine kinase B type
- SpecificityThis antibody is specific for Creatine kinase BB.
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
A KLH conjugated synthetic peptide selected within aa 200~300 of human Creatine Kinase BB.
- Positive controlY79 cell lysate and mouse colon tissue lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityProtein G purified
- Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab38211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/1000. |
| WB | WB: 1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Target
- FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
- Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. - Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 1152 Human
- Entrez Gene: 12709 Mouse
- Omim: 123280 Human
- SwissProt: P12277 Human
- SwissProt: Q04447 Mouse
- Unigene: 173724 Human
- Unigene: 16831 Mouse
Target information above from: UniProt accession
P12277
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- B CK antibodyB CK antibodyB-CK antibody
- BB-CK antibodyBCK antibodyBrain creatine kinase antibodyCkb antibodyCKBB antibodyCreatine kinase B antibodyCreatine kinase B chain antibodyCreatine kinase B type antibodyCreatine kinase B-type antibodyCreatine Kinase BB Isoenzyme antibodyCreatine Kinase BB Isoenzyme antibodyCreatine Kinase BB Isoenzyme antibodyCreatine kinase brain antibodyCreatine phosphokinase BB antibodyKCRB_HUMAN antibody
see all
Anti-Creatine kinase B type antibody images
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Western blot - Creatine Kinase BB antibody (ab38211)All lanes : Anti-Creatine kinase B type antibody (ab38211) at 1/100 dilution
Lane 1 : Y79 cell lysate
Lane 2 : Mouse colon tissue lysate
Predicted band size : 43 kDa
Observed band size : 43 kDa -
Western blot - Creatine Kinase BB antibody (ab38211)Anti-Creatine kinase B type antibody (ab38211) at 1/250 dilution + Tissue lysate prepared from human brain tissue at 10 µg
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/7500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 43 kDa
Exposure time : 1 minuteThis image is a courtesy of Anonymous Abreview
References for Anti-Creatine kinase B type antibody (ab38211)
This product has been referenced in:
- Mochel F et al. Early alterations of brain cellular energy homeostasis in huntington disease models. J Biol Chem 287:1361-70 (2012). WB ; Mouse . Read more (PubMed: 22123819) »
- Zhang SF et al. Impaired brain creatine kinase activity in Huntington's disease. Neurodegener Dis 8:194-201 (2011). Mouse . Read more (PubMed: 21124007) »
