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Creatine Kinase MB protein (Active) (ab73650)

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Overview

  • Product nameCreatine Kinase MB protein (Active)See all Creatine Kinase MB proteins and peptides ...
  • Protein descriptionHuman Recombinant Full Length Creatine Kinase MB, type 2, produced in Pichia Pastoris. Swiss IDs = P12277 and P06732.
  • Expression hostPichia pastoris

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab73650 is purified by proprietary chromatographic techniques under non-denaturing conditions. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • Biological activityThe biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 892 IU/mg at 37 degrees celsius.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 6.8
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab73650 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab151579.

    • Protein info

    • Alternative names
        B-CKbrain creatine kinaseCKBB
        CKMBCKMMcreatine kinase BCreatine kinase B chaincreatine kinase braincreatine kinase MCreatine kinase M chainCreatine kinase MBCreatine Kinase MB typecreatine kinase muscleM-CKmuscle creatine kinase
      see all
  • RelevanceCreatine Kinase MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Creatine Kinase MB reversibly catalyses the transfer of phosphate between ATP and various phosphogens. The creatine kinase isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands such as skeletal muscle, heart, brain and spermatozoa.
  • Cellular localizationCytoplasmic and Mitochondrial

    • References for Creatine Kinase MB protein (Active) (ab73650)

    ab73650 has not yet been referenced specifically in any publications.

    Publishing research using ab73650 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab73650 Submit a Question for ab73650

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

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    Our customer is going to test sELISA using ab19603-ab110655 or ab19603-ab87580 pair.
    He needs standard protein and let me know if ab73649 or ab72650 is okay. Please let me know which one is better choice.
    Best regards

    Thank you for contacting us.

    The protein ab73649 is Creatine Kinase MB Isoenzyme Type-I and protein ab73650 is Creatine Kinase MB, type 2. Theoratically both will work; customer can buy any of these.

    I hope this information is helpful to...

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    I'm sorry that preious mailwas wronged. Please check this.
    Our customer purchased ab31832 and ab19603 as capture and detection antibody of sandwich ELISA and he knows ab31832 is not guaranteed for sELISA.
    He would like to know if it is available...

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    Thank you for your email. Dr.Tanya is out of office so I am dealing with her inquiries.

    ab31832 and ab19603 has not been tested together in sandwich ELISA so I am sorry we will not be able to confirm it.

    We haven't tested ab73649 and a...

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    Dear technical team,
    Our customer purchased ab31832, ab619603 for sandwich ELISA.

    He found ab73649 and ab73650 to use as standard and would like to know if this application is possible.

    And he would like to receive immunogen inf...

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    Thank you for your enquiry and your interest in our products.

    I can confirm that ab31832 has been tested in ELISA (indirect ELISA) and but not in sandwich ELISA system. It seems that the other product code number provided - ab619603 - is incorre...

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    Dear technical team,

    Our customer is going to sELISA with Creatine Kinase MB antibodies.
    He wants full length immunogen and I searched ab11045 and ab404 as a monoclonal antibody.
    Let me know which one is meet the customer's needs.

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    Thank you for your enquiries and your interest.

    Currently, we only have two antibodies (ab19603 and ab110655) which have been tested for sELISA - and only ab19603 is monoclonal.

    Full length proteins are the followings: ab73649 and 7365...

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    Our customer raises an enquiry about ab109675 (Creatine Kinase MB protein), ab73649 (Creatine Kinase MB protein (Active)) and ab73650 (Creatine Kinase MB protein (Active)), this customer wants to conduct the ELISA assay with human sample, therefore, she w...

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    Thank you for contacting us. The tested pair of anti Creatine Kinase MB is ab19603 - ab110655. Please be advised that none of the two antibodies is conjugated so you may need a tertiary conjugated antibody for detection in sELISA. We suggest using mouse m...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"