Anti-Creatine Kinase MM antibody [ZM-1] (ab170173)
Key features and details
- Mouse monoclonal [ZM-1] to Creatine Kinase MM
- Suitable for: IP, WB
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
-
Product name
Anti-Creatine Kinase MM antibody [ZM-1]
See all Creatine Kinase MM primary antibodies -
Description
Mouse monoclonal [ZM-1] to Creatine Kinase MM -
Host species
Mouse -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Zebrafish
Does not react with: Mouse, Rat, Cow, Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Zebrafish Skeletal Muscle Homogenate.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
-
Purity
SDS-PAGE -
Clonality
Monoclonal -
Clone number
ZM-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
|
Notes |
---|
IP
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. |
Target
-
Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Alternative names
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
see all
Images
-
Immunoprecipitation for Creatine Kinase MM (ZM-1) antibody.
ab170173 conjugated to Protein-G-Beads, used at 20 µg antibody per 10 µL of beads. Immunoprecipitated from 1 mg Zebrafish Skeletal Muscle Homogenate Lysate. 25 µL eluate was loaded onto the gel.
Stained with Colloidal Coomassie Blue
-
All lanes : Anti-Creatine Kinase MM antibody [ZM-1] (ab170173) at 0.5 µg/ml
Lane 1 : ZBH (Zebrafish Brain Homogenate) Lysate
Lane 2 : (Zebrafish Skeletal Muscle Homogenate) Lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG –HRP at 1/2000 dilution
Predicted band size: 43 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab170173 has not yet been referenced specifically in any publications.