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Creatine Kinase MM protein (Active) (ab73652)

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Overview

  • Product nameCreatine Kinase MM protein (Active)See all Creatine Kinase MM proteins and peptides ...
  • Protein descriptionHuman Recombinant Full length Creatine Kinase MM, Type One (without a C terminal Lysine on both chains). A glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme. Produced in Pichia Pastoris, SwissProt P06732. (predicted M.Wt of monomer 43 kDa)
  • Expression hostPichia pastoris

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab73652 is purified by proprietary chromatographic techniques under non-denaturing conditions. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • Biological activityThe biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 500IU/mg at 37 degrees celsius.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 0.1M Tris HCl, 0.075M Sodium chloride, 10mM Beta mercaptoethanol, pH 7.2
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab73652 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        CKMCKMMCreatine kinase M
        Creatine kinase M chainCreatine kinase M typeCreatine kinase M-typeCreatine kinase muscleKCRM_HUMANM CKM-CKMCKMuscle creatine kinase
      see all
  • FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P06732 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Creatine Kinase MM protein (Active) (ab73652)

    ab73652 has not yet been referenced specifically in any publications.

    Publishing research using ab73652 ? Please let us know so that we can cite the reference in this datasheet

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    I know that the biological activity was measured as 500IU/mg at 37 degrees celsius. However, what is the maximum activity of the creatine kinase MM protein per mg?

    Thank you for your inquiry. The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 537IU/mg at 37 degrees celsius. I hope this information helps. Please let me kno...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"