Anti-Creatine kinase B type antibody [CK-BYK/21E10] (ab125114)
Key features and details
- Mouse monoclonal [CK-BYK/21E10] to Creatine kinase B type
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Creatine kinase B type antibody [CK-BYK/21E10]
See all Creatine kinase B type primary antibodies -
Description
Mouse monoclonal [CK-BYK/21E10] to Creatine kinase B type -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide:
SNSHNALKLRFPAEDEF
, corresponding to N terminal amino acids 4-20 of Human Creatine kinase B type. -
Positive control
- Human Purkinje cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CK-BYK/21E10 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/50. Detects a band of approximately 43-46 kDa (predicted molecular weight: 43 kDa).
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Notes |
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WB
1/50. Detects a band of approximately 43-46 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 516210 Cow
- Entrez Gene: 1152 Human
- Entrez Gene: 12709 Mouse
- Omim: 123280 Human
- SwissProt: Q5EA61 Cow
- SwissProt: P05124 Dog
- SwissProt: P12277 Human
- SwissProt: Q04447 Mouse
see all -
Alternative names
- B CK antibody
- B-CK antibody
- BB-CK antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab125114 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab125114 was shown to recognize Creatine kinase B when Creatine kinase B knockout samples were used, along with additional cross-reactive bands. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab125114 and ab181602 (loading control to GAPDH) were diluted 1/50 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW)preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Datasheets and documents
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Datasheet download
References (4)
ab125114 has been referenced in 4 publications.
- Wang Z et al. CKB inhibits epithelial-mesenchymal transition and prostate cancer progression by sequestering and inhibiting AKT activation. Neoplasia 23:1147-1165 (2021). PubMed: 34706306
- Narayan DS et al. Investigations Into Bioenergetic Neuroprotection of Cone Photoreceptors: Relevance to Retinitis Pigmentosa. Front Neurosci 13:1234 (2019). PubMed: 31803010
- Liu B et al. Proteome-wide analysis of USP14 substrates revealed its role in hepatosteatosis via stabilization of FASN. Nat Commun 9:4770 (2018). PubMed: 30425250
- Glover LE et al. Control of creatine metabolism by HIF is an endogenous mechanism of barrier regulation in colitis. Proc Natl Acad Sci U S A 110:19820-5 (2013). PubMed: 24248342