Overview

  • Product nameCreatinine Assay KitSee all Creatinine kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Product overview

    Abcam's Creatinine Assay Kit provides an accurate, convenient measure of creatine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm). Unlike picric acid assays, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Creatinine is a breakdown product of creatine phosphate. Creatinine is produced and excreted at a constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function. Blood creatinine levels increase only in cases of significant (>75%) damage to nephrons. Creatinine clearance is frequently used as a means of standardizing excretion of other compounds such as isoprostanes.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCreatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.

Applications

Our Abpromise guarantee covers the use of ab65340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

Creatinine Assay Kit images

  • Creatinine Standard Curve using ab65340. (X-axis showing standard absolute amount in nmol)

References for Creatinine Assay Kit (ab65340)

This product has been referenced in:
  • Yasuda K  et al. Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia. Am J Physiol Renal Physiol 302:F713-21 (2012). Mouse . Read more (PubMed: 22160777) »
  • Rabadi MM  et al. HMGB1 in renal ischemic injury. Am J Physiol Renal Physiol 303:F873-85 (2012). Read more (PubMed: 22759395) »

See all 6 Publications for this product

Product Wall

I have contacted the lab who have confirmed that they recommend Heparin over EDTA when preparing blood samples for use with this kit.

Please add background mix to standard and samples in separate wells? We recomend performing this in duplicate and then subtracting the mean from the original reading of standard and sample.

We recommend diluting the probe 10x and then using 0.4ul of the probe for the best results for the fluorometric assay. Typically this works the best. This assay is very sensitive and having too much probe will result in very high RFU values beyond the ...

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We have not tested this kit individually in higher than physiological amounts of the moieties you mentioned. In typical samples, to our knowledge sarcosine and creatine can interfere with the assay which is resolved by running appropriate controls. Sin...

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Gracias por contactarnos.
Este kit esta diseñado para mamíferos, y su límite de detección vendrá determinado por el valor más bajo de la lectura siempre que caiga dentro del rango linear de la recta estándar. Por ejemplo, el suero contiene entre 4...

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I have contacted the lab for additional advice.

My suggestions -as we discuss over the phone - would be:
1) check the creatine and sarcosine background
2) use serum instead of plasma
3) deproteinize the samples as recommended ...

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I can confirm that this kit can be used for measuring creatinine, not creatine kinase. (creatine kinase level is a marker for myocardial infarction).

However we are not specialized in this particular field and we cannot determine if...

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1. Yes, if you have access to a fluorometric plate reader, we would recommend using fluorometric analysis since it is in general ˜10 times more sensitive that the colorimetric.

2. You can use 0.4 µl probe for such analysis. Just increa...

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We have added the standard for ab65340 to our catalog under catalog number ab168168:
http://www.abcam.com/creatinine-standard-ab168168.html

Thank you for your enquiry regarding ab65340. I am very sorry to hear that you are having problems with this kit.

As I understand, the standard curve was fine and the values of the STD points provided the expected results. This implies that t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"