For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Abcam's Creatinine Assay Kit provides an accurate, convenient measure of creatine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm). Unlike picric acid assays, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.
Visit our FAQs page for tips and troubleshooting.
Creatinine is a breakdown product of creatine phosphate. Creatinine is produced and excreted at a constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function. Blood creatinine levels increase only in cases of significant (>75%) damage to nephrons. Creatinine clearance is frequently used as a means of standardizing excretion of other compounds such as isoprostanes.
|Creatinase (Lyophilized)||Blue||1 vial|
|Creatininase (Lyophilized)||Violet||1 vial|
|Creatinine (10 µmol) (Lyophilized)||Yellow||1 vial|
|Creatinine Assay Buffer||WM||1 x 25ml|
|Creatinine Enzyme Mix (Lyophilized)||Green||1 vial|
|Creatinine Probe in DMSO (200ul)||1 x 200µl|
Our Abpromise guarantee covers the use of ab65340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted.
Creatinine Standard Curve using ab65340. (X-axis showing standard absolute amount in nmol)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"