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Overview

  • Product nameCreatinine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Product overview

    Abcam's Creatinine Assay Kit provides an accurate, convenient measure of creatine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm). Unlike picric acid assays, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Creatinine is a breakdown product of creatine phosphate. Creatinine is produced and excreted at a constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function. Blood creatinine levels increase only in cases of significant (>75%) damage to nephrons. Creatinine clearance is frequently used as a means of standardizing excretion of other compounds such as isoprostanes.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCreatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.

    • Applications

    Our Abpromise guarantee covers the use of ab65340 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Creatinine Assay Kit images

    • Functional Studies - Creatinine Assay Kit (ab65340)
      Functional Studies - Creatinine Assay Kit (ab65340)

      Creatinine Standard Curve using ab65340. (X-axis showing standard absolute amount in nmol)

    Protocols

    References for Creatinine Assay Kit (ab65340)

    This product has been referenced in:
    • Yasuda K  et al. Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia. Am J Physiol Renal Physiol 302:F713-21 (2012). Mouse . Read more (PubMed: 22160777) »
    • Rabadi MM  et al. HMGB1 in renal ischemic injury. Am J Physiol Renal Physiol 303:F873-85 (2012). Read more (PubMed: 22759395) »

    See all 6 Publications for this product

    Publishing research using ab65340 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65340 Submit a Question for ab65340

    Displaying 1 - 10 of 45 results for Abreviews and Q&A

    10
    Votes: Highest First

    uses mouse plasma (20 ul)
    good standard curve

    values of creatinine obtained is 10x higher than what is reported in literature
    uses 2.5x dilution
    obtained 2.2 mg/dL = 200 pmol / uL (Note in protocol: Serum contains ˜45-110 pmol/μl ...

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    I have contacted the lab for additional advice.

    My suggestions -as we discuss over the phone - would be:
    1) check the creatine and sarcosine background
    2) use serum instead of plasma
    3) deproteinize the samples as recommended in ...

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    Product code: 65340

    Inquiry: May i know if the creatinine assay kit, product code-65430 can be used for acute myocardial infarction in rat serum and tissue homogenate or is it used only for ischemic kidney?



    I can confirm that this kit can be used for measuring creatinine, not creatine kinase. (creatine kinase level is a marker for myocardial infarction).

    However we are not specialized in this particular field and we cannot determine if cr...

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    I will use this kit to measure mice creatinine, which normally runs around 0.15mg/dl.

    1) Do you recommend me to use fluorometric measurement instead of colorimetric measurement because the concentration of creatinine is too low?

    2) O...

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    1. Yes, if you have access to a fluorometric plate reader, we would recommend using fluorometric analysis since it is in general ˜10 times more sensitive that the colorimetric.

    2. You can use 0.4 µl probe for such analysis. Just increase ...

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    Standard is only stable for 2 months while the other kit components are stable for longer. Thus, in order to increase the longevity of the kit, is there a way to either:
    1. Purchase the standard separately
    2. Reconstitute only a portion of the lyo...

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    We have added the standard for ab65340 to our catalog under catalog number ab168168:
    http://www.abcam.com/creatinine-standard-ab168168.html

    To whom it may concern:
    I purchased your kit ab65340 Creatinine Assay kit (GR111057-1) from
    your NZ distributor Sapphire Bioscience and used it yesterday to test
    serum samples for creatinine concentration, ending up with unexpected
    low...

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    Thank you for your enquiry regarding ab65340. I am very sorry to hear that you are having problems with this kit.

    As I understand, the standard curve was fine and the values of the STD points provided the expected results. This implies that the ...

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    Inquiry: Hello Can I use creatinine assay kit (ab65340) for detection creatinin levels in rat urine. Thanks

    Thank you for your enquiry.

    I can confirm that the ab65340 Creatinine Assay Kit should theoretically be compatible with rat urine. Please note that we have not specifically tested the kit with rat urine in the laboratory. However, the assay is ...

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    Inquiry: HI I tried to measure creatinine level in mice,and estimated creatinine is 0.2-0.5mg/dl. I calculated the concentration and found that 0.2-0.5mg/dl will be at the bottom of the standard curve. I understand it is better to have sample concentratio...

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    Thank you for contacting Abcam.

    The sensitivity of the kit can be increased by following the information provided in the protocol under section 4.b.:
    " If a more sensitive assay is desired, fluorescence can be utilized. Dilute the standa...

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    Customer kindly contacted us regarding a missing component in ab65340.

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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    Can this kit be used with whole blood prepared with heparin, or will this interfere with the assay? Samples are too small (50-100 uL of whole blood from rats) to prepare serum or plasma.

    Thank you very much for your call yesterday and for your patience while I've been in touch with the lab regarding your enquiry.

    We have only tested this kit using plasma and serum samples, and we aren't sure that whole blood will work in the kit...

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    I would like to use your Creatinine Assay Kit (ab65340) to look at the hydrolysis of creatinine in bacterial cultures grown in artificial intestinal fluid. I would appreciate your comments on the application of the kit for this purpose.

    Thank you for contacting Abcam regarding ab65340.


    I have consulted with the laboratory and unfortunately we have not used this kit with samples from bacteria. It is possible that the kit will work, but would need to be optimized with your sa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"