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Product name
Anti-Cripto1 antibody - Embryonic Stem Cell Marker
See all Cripto1 products (7) ...
Description
Rabbit polyclonal to Cripto1 - Embryonic Stem Cell Marker
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Cow, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Cripto.
(Peptide available as ab233 10.)
Positive control
Human Embryonic Stem Cells, Cripto1 Recombinant Protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Embryogenesis >> Morphogens
Stem Cells >> Embryonic Stem Cells >> Secreted
Stem Cells >> Lineage Markers >> Ectoderm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at a concentration of 0.5 - 2.5 ug/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 21 kDa). Can be blocked with Cripto peptide (ab23310).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Tissue specificity
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Sequence similarities
Contains 1 EGF-like domain.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP13385
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CR antibody
- CRGF antibody
- Cripto 1 antibody
see all
Database links
- Entrez Gene: 784029 Cow
- Entrez Gene: 6997 Human
- Entrez Gene: 21667 Mouse
- Entrez Gene: 57932 Zebrafish
- Omim: 187395 Human
- SwissProt: Q58D57 Cow
- SwissProt: P13385 Human
- SwissProt: P51865 Mouse
see all
Anti-Cripto1 antibody - Embryonic Stem Cell Marker images:
Immunocytochemistry/ Immunofluorescence - Cripto1 antibody - Embryonic Stem Cell Marker (ab19917)
ICC/IF image of ab19917 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Cripto antibody - Embryonic Stem Cell Marker (ab19917)
All lanes : Anti-Cripto1 antibody - Embryonic Stem Cell Marker (ab19917) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate at 20 µg
Lane 2 : Cripto protein at 0.1 µg
Lane 3 : Human Embryonic Stem Cell Lysate at 20 µg with
Lane 4 : Cripto protein at 0.1 µg with
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Predicted band size : 21 kDa
Observed band size : 17,17-21 kDa (why is the actual band size different from the predicted?)
The 17 kDa band detected in human embryonic stem cells may correspond with Cripto that has had its signal sequence cleaved. The Cripto bands were blocked with the immunizing peptide, ab23310.
Immunocytochemistry/ Immunofluorescence - Cripto antibody - Embryonic Stem Cell Marker (ab19917)
Anti-Cripto antibody, ab19917, stained the membrane of human embryonic stem cells at a dilution of 1/100.
Ludovic Vallier, University of Cambridge, UK
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cripto antibody - Embryonic Stem Cell Marker (ab19917)
The image shows staining of human pancreas tissue. Staining was nuclear in some islet cells and cytoplasmic in some cells of the acini. Antigen retrieval was performed using Citrate at pH6. Nuclear localization of Cripto protein has been described in the literature: Gong YP et al. Eur J Surg Oncol, 2006.
References for Anti-Cripto1 antibody - Embryonic Stem Cell Marker (ab19917)
This product has been referenced in:
- Williams TA et al. Teratocarcinoma-derived growth factor-1 is upregulated in aldosterone-producing adenomas and increases aldosterone secretion and inhibits apoptosis in vitro. Hypertension 55:1468-75 (2010).Read more (PubMed: 20385969) »
- Lu B et al. Long-term safety and function of RPE from human embryonic stem cells in preclinical models of macular degeneration. Stem Cells 27:2126-35 (2009). WB; Human.Read more (PubMed: 19521979) »
See all 3 publications for this product
Publishing research using ab19917? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"