Cripto1 protein (Active) (ab84062)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab84062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Under reducing conditions ab84062 migrates as a broad band between 45 and 55 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified protein that has a predicted monomeric molecular mass of 42.8 kDa. ab84062 consists of 5-30% carbohydrate by weight.
ab84062 separates into a number of glycoforms with an observed pI between 6.5 and 10 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 8.2.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Cripto 1Cripto 1 growth factorCripto-1 growth factorCripto1Cripto1 growth factorEpidermal growth factor like cripto protein CR1Epidermal growth factor-like cripto protein CR1TDGF 1TDGF1TDGF1_HUMANTeratocarcinoma derived growth factor 1Teratocarcinoma-derived growth factor 1
Cripto1 protein (Active) images
Lane 1 – MW markers; Lane 2 – ab84062; Lane 3 – ab84062 treated with PNGase F to remove potential N linked glycans; Lane 4 – ab84062 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
A sample of ab84062 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4 20% Tris-HCl 2D gel. Approximately 40 µg of protein was loaded; Gel was stained using Deep Purple™. The spot train indicates the presence of multiple glycoforms. Spots within the spot train were cut from the gel and identified as Cripto1 (Fc Chimera) by protein mass fingerprinting.
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification. The triangle indicates theoretical pI and MW of the protein.
References for Cripto1 protein (Active) (ab84062)
ab84062 has not yet been referenced specifically in any publications.