Recombinant Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR269Y] to Crk p38 (phospho Y221)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Crk p38 (phospho Y221) antibody [EPR269Y]
See all Crk p38 primary antibodies -
Description
Rabbit monoclonal [EPR269Y] to Crk p38 (phospho Y221) -
Host species
Rabbit -
Specificity
This antibody detects proteins phosphorylated at tyrosine 221. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR269Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 34 kDa.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 34 kDa. |
Target
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Function
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. -
Sequence similarities
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
The SH2 domain mediates interaction with SHB. -
Post-translational
modificationsPhosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. -
Cellular localization
Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion. - Information by UniProt
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Database links
- Entrez Gene: 1398 Human
- Omim: 164762 Human
- SwissProt: P46108 Human
- Unigene: 638121 Human
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Alternative names
- Adapter molecule crk antibody
- Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
- CRK antibody
see all
Images
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All lanes : Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227) at 1/2000 dilution
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab76227 has been referenced in 5 publications.
- Zeng HT et al. Modulating Oxidative Stress in B Cells Promotes Immunotherapy in Food Allergy. Oxid Med Cell Longev 2022:3605977 (2022). PubMed: 35096267
- Nieto-Pelegrin E et al. Crk adaptors negatively regulate actin polymerization in pedestals formed by enteropathogenic Escherichia coli (EPEC) by binding to Tir effector. PLoS Pathog 10:e1004022 (2014). WB ; Human . PubMed: 24675776
- Li XN et al. The p38 MAPK inhibitor JLU1124 inhibits the inflammatory response induced by lipopolysaccharide through the MAPK-NF-?B pathway in RAW264.7 macrophages. Int Immunopharmacol 17:785-92 (2013). PubMed: 24070708
- Ferrando IM et al. Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics. Mol Cell Proteomics 11:355-69 (2012). WB . PubMed: 22499769
- Torres MP et al. Effects of thymoquinone in the expression of mucin 4 in pancreatic cancer cells: implications for the development of novel cancer therapies. Mol Cancer Ther 9:1419-31 (2010). PubMed: 20423995