CrkL antibody [Y244] (ab32018)

Product Name 

CrkL antibody [Y244]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y244] to CrkL

Immunogen 

Synthetic peptide corresponding to residues in the second SH3 domain and near C terminus of human Crk like protein

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

ab32018 recognises CrkL (Crk-like)protein.

Tested applications 
(see key)

ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/50.
IHC-P: 1/50.
IP: 1/10.
WB: 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

K562 cell lysate, human metastatic lung carcinoma

Cellular localization 

Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

Relevance 

Crkl is a 33kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y244

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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