![Western blot - CrkL antibody [Y244] (ab32018) image](#ab32018_1.JPG)
![Immunohistochemistry (Paraffin-embedded sections) - CrkL antibody [Y244] (ab32018) image](#ab32018_2.JPG)
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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-CrkL antibody [Y244]
See all CrkL products (8) ...
Description
Rabbit monoclonal [Y244] to CrkL
Specificity
ab32018 recognises CrkL (Crk-like)protein.
Tested applications
WB, IHC-P, ICC, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues in the second SH3 domain and near C terminus of human Crk like protein
Positive control
K562 cell lysate, human metastatic lung carcinoma
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
Y244
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
IHC-P: 1/50
ICC
ICC: 1/50ICC: 1/50
IP
IP: 1/10IP: 1/10
Target
Function
May mediate the transduction of intracellular signals.
Sequence similarities
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Target information above from: UniProt accessionP46109
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
see all
Database links
- Entrez Gene: 1399 Human
- Omim: 602007 Human
- SwissProt: P46109 Human
- Unigene: 5613 Human
Anti-CrkL antibody [Y244] images:
Western blot - CrkL antibody [Y244] (ab32018)
![Western blot - CrkL antibody [Y244] (ab32018)](/ps/datasheet/Images/32/ab32018/ab32018_1.JPG)
Anti-CrkL antibody [Y244] (ab32018) at 1/500 dilution + K562 cell lysate
Predicted band size : 33 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - CrkL antibody [Y244] (ab32018)
![Immunohistochemistry (Paraffin-embedded sections) - CrkL antibody [Y244] (ab32018)](/ps/datasheet/Images/32/ab32018/ab32018_2.JPG)
ab32018, at a 1/50 dilution, staining human Crk in metastatic lung carcinoma by Immunohistochemistry, in paraffin embedded tissue
References for Anti-CrkL antibody [Y244] (ab32018)
ab32018 has not yet been referenced specifically in any publications.
Publishing research using ab32018? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"