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This image shows HuvEc cells stained with ab63491 at 1/500 dilution; with and without the immunising peptide.
This image shows human lung carcinoma tissue stained with ab63491 at 1/50 dilution; with and without the immunising peptide.
All lanes : Anti-CrkL antibody (ab63491) at 1/300 dilution
Lane 1 : Extracts from 293 cells (5-30µg total protein)
Lane 2 : Extracts from 293 cells (5-30µg total protein) and the immunising peptide (5-10µg).
Predicted band size : 34 kDa
Observed band size : 34 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
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