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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-CrkL antibody
See all CrkL products (8) ...
Description
Rabbit polyclonal to CrkL
Tested applications
WB, IHC-P, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic non-phosphopeptide derived from human CrkL around the phosphorylation site of tyrosine 207 (H-A-YP-A-Q).
Positive control
Human lung carcinoma tissue, extracts from 293 cells, HuvEc cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/1000.Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10,000ELISA: 1/10,000
ICC/IF: 1/500 - 1/1000.
Target
Function
May mediate the transduction of intracellular signals.
Sequence similarities
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Target information above from: UniProt accessionP46109
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
see all
Database links
- Entrez Gene: 1399 Human
- Entrez Gene: 12929 Mouse
- Omim: 602007 Human
- SwissProt: P46109 Human
- SwissProt: P47941 Mouse
- Unigene: 5613 Human
- Unigene: 21048 Mouse
- Unigene: 451241 Mouse
Anti-CrkL antibody images:
Immunocytochemistry/ Immunofluorescence - CrkL antibody (ab63491)
This image shows HuvEc cells stained with ab63491 at 1/500 dilution; with and without the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CrkL antibody (ab63491)
This image shows human lung carcinoma tissue stained with ab63491 at 1/50 dilution; with and without the immunising peptide.
Western blot - CrkL antibody (ab63491)
All lanes : Anti-CrkL antibody (ab63491) at 1/300 dilution
Lane 1 : Extracts from 293 cells (5-30µg total protein)
Lane 2 : Extracts from 293 cells (5-30µg total protein) and the immunising peptide (5-10µg).
Predicted band size : 34 kDa
Observed band size : 34 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
References for Anti-CrkL antibody (ab63491)
ab63491 has not yet been referenced specifically in any publications.
Publishing research using ab63491? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"