![Western blot - CrkL antibody [EP270Y] (ab52908) image](#ab52908_wb.jpg)
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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-CrkL (phospho Y207) antibody [EP270Y]
See all CrkL products (8) ...
Description
Rabbit monoclonal [EP270Y] to CrkL (phospho Y207)
Specificity
ab52908 only detects CrkL phosphorylated on Tyrosine 207.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
A synthetic phospho peptide corresponding to residues surrounding Tyr207 of human CrkL
Positive control
WB: K562 cell lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP270Y
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Western blot - CrkL antibody [EP270Y] (ab52908)
![Western blot - CrkL antibody [EP270Y] (ab52908) image](/ps/datasheet/Images/52/ab52908/ab52908_wb.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50000Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).
IP
IP: 1/50IP: 1/50
Application notes
Is unsuitable for ,Flow Cyt or IHC-P.
Target
Function
May mediate the transduction of intracellular signals.
Sequence similarities
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Target information above from: UniProt accessionP46109
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
see all
Database links
- Entrez Gene: 1399 Human
- Entrez Gene: 12929 Mouse
- Omim: 602007 Human
- SwissProt: P46109 Human
- SwissProt: P47941 Mouse
- Unigene: 5613 Human
- Unigene: 21048 Mouse
- Unigene: 451241 Mouse
Anti-CrkL (phospho Y207) antibody [EP270Y] images:
Western blot - CrkL antibody [EP270Y] (ab52908)
All lanes : Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution
Lane 1 : (A) K562 cell lysates untreated
Lane 2 : (B) K562 cell lysates treated with AP
Lysates/proteins at 10 µg per lane.
Predicted band size : 34 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
References for Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)
This product has been referenced in:
- Placzek EAet al. A peptide biosensor for detecting intracellular Abl kinase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Biochem 397:73-8 (2010). WB; Human.Read more (PubMed: 19818327) »
See 1 publication for this product
Publishing research using ab52908? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"