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Western blot - CrkL antibody [EP270Y] (ab52908)
![Western blot - CrkL antibody [EP270Y] (ab52908)](/ps/datasheet/images/ab52908_wb.jpg)
(enlarge)
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Product Name
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CrkL (phospho Y207) antibody [EP270Y]
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See all CrkL antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP270Y] to CrkL (phospho Y207)
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Immunogen
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A synthetic phospho peptide corresponding to residues surrounding Tyr207 of human CrkL
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Reacts with
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Hu, Ms
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Does not react with
(species key)
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Rat
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Specificity
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ab52908 only detects CrkL phosphorylated on Tyrosine 207.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: 1/50.
WB: 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).
Is unsuitable for Flow Cyt or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: K562 cell lysates
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Relevance
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Crkl is a 33kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.
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CrkL (phospho Y207) antibody [EP270Y] (ab52908) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP270Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CrkL (phospho Y207) antibody [EP270Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CrkL (phospho Y207) antibody [EP270Y] images, references, products related to ab52908 and other tools.
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CrkL (phospho Y207) antibody [EP270Y] images:
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 Western blot - CrkL antibody [EP270Y] (ab52908)
All lanes : CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution
Lane 1 : (A) K562 cell lysates untreated
Lane 2 : (B) K562 cell lysates treated with AP
Lysates/proteins at 10 µg per lane.
Predicted band size : 34 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
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References for CrkL (phospho Y207) antibody [EP270Y] (ab52908)
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This product has been used in:
Placzek EA et al. A peptide biosensor for detecting intracellular Abl kinase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Biochem 397:73-8 (2010). WB; Human. PubMed: 19818327
If you publish research using ab52908 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CrkL
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CrkL (phospho Y207) antibody [EP270Y] - more information
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Customer reviews (feedback) regarding CrkL (phospho Y207) antibody [EP270Y] |
Customer FAQs regarding CrkL (phospho Y207) antibody [EP270Y] |
Protocols for CrkL (phospho Y207) antibody [EP270Y] |
Price and availability of products related to CrkL (phospho Y207) antibody [EP270Y] |
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CrkL (phospho Y207) antibody [EP270Y] - related products:
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CrkL (phospho Y207) antibody [EP270Y] - other tools:
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Contact Abcam with a Technical Enquiry about CrkL (phospho Y207) antibody [EP270Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - CrkL antibody [EP270Y] (ab52908)](/index.html?datasheet=52908#ab52908_wb.jpg)
