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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-CrkL (phospho Y207) antibody
See all CrkL products (8) ...
Description
Rabbit polyclonal to CrkL (phospho Y207)
Specificity
This antibody detects endogenous levels of Crkl only when phosphorylated at tyrosine 207.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
A synthesized phosphopeptide derived from human CrkL around the phosphorylation site of tyrosine 207(H-A-YP-A-Q).
Positive control
Extracts of cos7 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Western blot - CrkL (phospho Y207) antibody (ab51225)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/1000.Detects a band of approximately 41 kDa (predicted molecular weight: 34 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
May mediate the transduction of intracellular signals.
Sequence similarities
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Target information above from: UniProt accessionP46109
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Crk L antibody
- Crk like protein antibody
- Crk-like protein antibody
see all
Database links
- Entrez Gene: 1399 Human
- Entrez Gene: 12929 Mouse
- Omim: 602007 Human
- SwissProt: P46109 Human
- SwissProt: P47941 Mouse
- Unigene: 5613 Human
- Unigene: 21048 Mouse
- Unigene: 451241 Mouse
Anti-CrkL (phospho Y207) antibody images:
Western blot - CrkL (phospho Y207) antibody (ab51225)
All lanes : Anti-CrkL (phospho Y207) antibody (ab51225) at 1/300 dilution
Lane 1 : Extracts of cos7 cells.
Lane 2 : Extracts of cos7 cells treated with phosphopeptide.
Predicted band size : 34 kDa
References for Anti-CrkL (phospho Y207) antibody (ab51225)
ab51225 has not yet been referenced specifically in any publications.
Publishing research using ab51225? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"