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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Overview
Product name
Anti-CtIP antibody
See all CtIP products (6) ...
Description
Rabbit polyclonal to CtIP
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CtIP.
(Peptide available as ab38676.)
Positive control
This antibody gave a positive signal in the following Human lysates: Jurkat Whole Cell HeLa Nuclear HepG2 Whole Cell HepG2 Nuclear F9 Whole Cell (data not shown) Y-79 Nuclear Lysate (data not shown) Caco 2 Whole Cell (data not shown)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 110 kDa (predicted molecular weight: 101 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Endonuclease that cooperates with the MRN complex in processing meiotic and mitotic double-strand breaks by ensuring both resection and intrachromosomal association of the broken ends. Promotes ATR and RPA recruitment to DSBs in S/G2 phase and facilitates the generation of ssDNA. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control.
Sequence similarities
Belongs to the COM1/SAE2/CtIP family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylated at Thr-847 by CDK1, which is essential for the recruitment to DNA and DNA repair function.
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Cellular localization
Nucleus. Associates with sites of DNA damage in S/G2 phase.
Target information above from: UniProt accessionQ99708
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COM1_HUMAN antibody
- CtBP interacting protein antibody
- CtBP-interacting protein antibody
see all
Database links
- Entrez Gene: 5932 Human
- Entrez Gene: 225182 Mouse
- Omim: 604124 Human
- SwissProt: Q99708 Human
- SwissProt: Q80YR6 Mouse
- Unigene: 546282 Human
- Unigene: 154275 Mouse
Anti-CtIP antibody images:
Western blot - CtIP antibody (ab38016)
All lanes : Anti-CtIP antibody (ab38016) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 101 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 25 kDa,250 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - CtIP antibody (ab38016)
ICC/IF image of ab38016 stained MCF-7 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38016 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 1µg/ml.
References for Anti-CtIP antibody (ab38016)
ab38016 has not yet been referenced specifically in any publications.
Publishing research using ab38016? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"