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Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

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Overview

Product name

Anti-Ctip2 antibody [25B6] - ChIP Grade
See all Ctip2 products (7) ...

Description

Rat monoclonal [25B6] to Ctip2 - ChIP Grade

Specificity

Detects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.

Tested applications

ICC/IF, IHC-FoFr, WB, IP, IHC-P, IHC-Fr, ChIP, IHC-FrFlmore details

Cross reactivity

Reacts with

Mouse, Human, Zebrafish

Immunogen

Fusion protein (Human).

Epitope

Between amino acids 1-150 of CTIP2.

Positive control

WB: nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody IHC: adult mouse hippocampus, Adult mouse spinal cord ICC: Neonatal mouse hippocampal cultured neurons

General notes

Hybridoma produced by fusion of a rat lymphocyte and mouse myeloma.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 150mM Sodium chloride, 15mM HEPES, pH 7.5

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

25B6

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Immunohistochemistry (Frozen sections) - Ctip2 antibody [25B6] - ChIP Grade (ab18465)Immunohistochemistry (Frozen sections) - Ctip2 antibody [25B6] - ChIP Grade (ab18465) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465) image (enlarge)

  • - Ctip2 antibody [25B6] (ab18465)- Ctip2 antibody [25B6] (ab18465) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab18465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.

Tissue specificity

Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.

Sequence similarities

Contains 6 C2H2-type zinc fingers.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9C0K0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • B cell lymphoma/leukemia 11B antibody
  • B-cell CLL/Lymphoma 11B antibody
  • B-cell lymphoma/leukemia 11B antibody
  • BC11B_HUMAN antibody
  • BCL-11B antibody
  • Bcl11B antibody
  • COUP-TF interacting protein 2 antibody
  • COUP-TF-interacting protein 2 antibody
  • Ctip 2 antibody
  • hRit1 antibody
  • MS1029 antibody
  • Radiation induced tumor suppressor gene 1 antibody
  • Radiation induced tumor suppressor gene 1 protein antibody
  • Radiation-induced tumor suppressor gene 1 protein antibody
  • Rit 1 antibody
  • Rit1 antibody
see all

Database links

Anti-Ctip2 antibody [25B6] - ChIP Grade images:

  Immunohistochemistry (Frozen sections) - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

Immunohistochemistry (Frozen sections) - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

ab18465 staining Ctip2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG (1/1000) was used as secondary antibody. Staining was improved with citrate antigen retrieval.

This image is courtesy of an anonymous Abreview

See Abreview

  Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).

Red is beta tubulin staining.
Green is ab18465.

  - Ctip2 antibody [25B6] (ab18465)

- Ctip2 antibody [25B6] (ab18465)

Immunohistochemistry using ab18465 on Adult Mouse Hippocampus.

  Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.

  Western blot - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

Western blot - Ctip2 antibody [25B6] - ChIP Grade (ab18465)



Predicted band size : 95 kDa


Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

  Western blot - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

Western blot - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

All lanes : Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465) at 1/500 dilution

Lane 1 : Mouse brain tissue lysate at 1.5 µg
Lane 2 : Mouse brain tissue lysate at 1.5 µg
Lane 3 : Mouse brain tissue lysate at 3 µg

Secondary
IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 95 kDa
Observed band size : 100,110 kDa (why is the actual band size different from the predicted?)


Exposure time : 10 minutes

This image is courtesy of an Anonymous Abreview.

See Abreview

References for Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

This product has been referenced in:

  • Jeong SJ  et al. Loss of Col3a1, the gene for Ehlers-Danlos syndrome type IV, results in neocortical dyslamination. PLoS One 7:e29767 (2012). IHC-FoFr; Mouse.Read more (PubMed: 22235340) »
  • Marinaro C  et al. In Vivo Fate Analysis Reveals the Multipotent and Self-Renewal Features of Embryonic AspM Expressing Cells. PLoS One 6:e19419 (2011). IHC-FoFr; Mouse.Read more (PubMed: 21559369) »

See all 40 publications for this product

Publishing research using ab18465? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"