Anti-Ctip2 antibody (ab70453)

Overview

• Product nameAnti-Ctip2 antibodySee all Ctip2 primary antibodies ...
• Description
  Rabbit polyclonal to Ctip2
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic peptide corresponding to a region between C terminal residues 850-894 of human Ctip2 (Swiss-Prot Q9C0K0).

• Positive controlNuclear extract from Jurkat cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HAT

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

Target

• FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
• Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
• Sequence similaritiesContains 6 C2H2-type zinc fingers.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9C0K0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 64919 Human
  • Entrez Gene: 58208 Mouse
  • Omim: 606558 Human
  • SwissProt: Q9C0K0 Human
  • SwissProt: Q99PV8 Mouse
  • Unigene: 709690 Human
  • Unigene: 392694 Mouse

• Alternative names
  B cell lymphoma/leukemia 11B antibodyB-cell CLL/Lymphoma 11B antibodyB-cell lymphoma/leukemia 11B antibody

  BC11B_HUMAN antibodyBCL-11B antibodyBcl11B antibodyCOUP-TF interacting protein 2 antibodyCOUP-TF-interacting protein 2 antibodyCtip 2 antibodyhRit1 antibodyRadiation induced tumor suppressor gene 1 antibodyRadiation induced tumor suppressor gene 1 protein antibodyRadiation-induced tumor suppressor gene 1 protein antibodyRit 1 antibodyRit1 antibody

  see all

Anti-Ctip2 antibody images

• 

  Immunoprecipitation - Ctip2 antibody (ab70453)
  All lanes: 1mg nuclear extract from Jurkat cells analysed on a blot using ab70453 at 0.5µg/ml.
  Lane 1: an alternate Ctip2 antibody used at 1 µg/mg lysate for IP.
  Lane 2: The same alternate antibody used at 10 µg/mg lysate for IP.
  Lane 3: ab70453 used at 1 µg/mg lysate for IP.
  Lane 4: ab70453 at 10 µg/mg lysate for IP.
  Normal rabbit IgG was used as a control for immunoprecipitation reactions.
• 

  Western blot - Ctip2 antibody (ab70453)
  All lanes : Anti-Ctip2 antibody (ab70453) at 0.1 µg/ml
  
  Lane 1 : Jurkat cell nuclear extract at 5 µg
  Lane 2 : Jurkat cell nuclear extract at 50 µg
  
  
  Predicted band size : 100 kDa
  Observed band size : 120-130 kDa (why is the actual band size different from the predicted?)