Recombinant Anti-Cubilin antibody [EPR16259-34] - C-terminal (ab191074)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16259-34] to Cubilin - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cubilin antibody [EPR16259-34] - C-terminal
See all Cubilin primary antibodies -
Description
Rabbit monoclonal [EPR16259-34] to Cubilin - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal kidney lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16259-34 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/40.
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WB |
1/1000 - 1/2000. Detects a band of approximately 399 kDa (predicted molecular weight: 399 kDa).
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Notes |
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IP
1/40. |
WB
1/1000 - 1/2000. Detects a band of approximately 399 kDa (predicted molecular weight: 399 kDa). |
Target
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Function
Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. -
Tissue specificity
Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney. -
Involvement in disease
Recessive hereditary megaloblastic anemia 1 -
Sequence similarities
Contains 27 CUB domains.
Contains 7 EGF-like domains. -
Domain
The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2. -
Post-translational
modificationsThe precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off.
N-glycosylated. -
Cellular localization
Endosome membrane. Lysosome membrane. Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 8029 Human
- Omim: 602997 Human
- SwissProt: O60494 Human
- Unigene: 166206 Human
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Alternative names
- 460 kDa receptor antibody
- cubilin (intrinsic factor-cobalamin receptor) antibody
- Cubilin antibody
see all
Images
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Anti-Cubilin antibody [EPR16259-34] - C-terminal (ab191074) at 1/5000 dilution + Human fetal kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 399 kDa
Observed band size: 399 kDa -
Western blot analysis of Cublin in immunoprecipitation pellets from Human fetal kidney lysate. ab191074 used at 1/40 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191074 has not yet been referenced specifically in any publications.