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My question is which peptides did you use to raise this antibody? Were they from the N-terminal? Many thanks, |
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ANSWER: |
Thank you for your enquiry. The peptide used to raise this Cullin 1 antibody is from the C-terminal region, within the last 20 aa. Note that this is a general region of the immunogen sequence and the exact sequence information is proprietary. I hope this information will still be helpful. If there is anything else that I can help you with, please do not hesitate to contact me. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab75817 at 1/100 dilution staining Cullin 1 in human cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
All lanes : Anti-Cullin 1 antibody [EPR3103Y] (ab75817) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 90 kDa
Observed band size : 90 kDa
ab75817 showing positive staining in Normal tonsil tissue.
ab75817 showing positive staining in Urinary bladder transitional carcinoma tissue.
ab75817 showing positive staining in Ovarian carcinoma tissue.
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