![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody [EPR3103Y] (ab75817) image](#ab75817ihc.gif)
![Western blot - Cullin 1 antibody [EPR3103Y] (ab75817) image](#ab75817wb.gif)
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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-Cullin 1 antibody [EPR3103Y]
See all Cullin 1 products (9) ...
Description
Rabbit monoclonal [EPR3103Y] to Cullin 1
Tested applications
WB, IP, IHC-P, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues on the C-terminus of human Cullin 1
Positive control
HeLa, MCF7, A549 and PC12 cell lysates; human cervical carcinoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR3103Y
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2500 - 1/5000.Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IP
IP: 1/10IP: 1/10
IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
Tissue specificity
Expressed in lung fibroblasts.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the cullin family.
Post-translational
modifications
Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
Target information above from: UniProt accessionQ13616
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CUL 1 antibody
- CUL-1 antibody
- CUL1 antibody
see all
Database links
- Entrez Gene: 8454 Human
- Entrez Gene: 26965 Mouse
- Entrez Gene: 362356 Rat
- Omim: 603134 Human
- SwissProt: Q13616 Human
- SwissProt: Q9WTX6 Mouse
- Unigene: 146806 Human
- Unigene: 87611 Mouse
see all
Anti-Cullin 1 antibody [EPR3103Y] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody [EPR3103Y] (ab75817)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody [EPR3103Y] (ab75817)](/ps/datasheet/Images/75/ab75817/ab75817ihc.gif)
ab75817 at 1/100 dilution staining Cullin 1 in human cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Western blot - Cullin 1 antibody [EPR3103Y] (ab75817)
![Western blot - Cullin 1 antibody [EPR3103Y] (ab75817)](/ps/datasheet/Images/75/ab75817/ab75817wb.gif)
All lanes : Anti-Cullin 1 antibody [EPR3103Y] (ab75817) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 90 kDa
Observed band size : 90 kDa
References for Anti-Cullin 1 antibody [EPR3103Y] (ab75817)
ab75817 has not yet been referenced specifically in any publications.
Publishing research using ab75817? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"