Recommended dilutions IHC-P: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, WB: 1/500. (PMID 19056892)
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Cullin 1 may be involved in the degradation of positive cell cycle regulators (for example G1 cyclins in yeast). Human cullin 1 forms a complex with Skp1 and the F box protein Skp2, which is thought to play a role in the ubiquitination of G1 regulatory proteins, as its homolog does in yeast.
Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
Form
Liquid
Storage instructions
Store at +4°C.
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cullin 1 antibody is on this datasheet. But please do contact us if you would like any reassurance!
References for Cullin 1 antibody (ab1868)
This product has been used in:
Miranda-Carboni GA et al. A functional link between Wnt signaling and SKP2-independent p27 turnover in mammary tumors. Genes Dev22:3121-34 (2008). WB; Human.PubMed: 19056892
If you publish research using ab1868 please let us know so that we can cite the reference on this datasheet.
Pricing information loading
This product has been used in: