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Anti-Cullin 1 antibody (ab53049)

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Overview

Product name

Anti-Cullin 1 antibody
See all Cullin 1 products (9) ...

Description

Rabbit polyclonal to Cullin 1

Specificity

ab53049 detects endogenous levels of total Cullin 1 protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

A synthetic peptide derived from human Cullin 1.

Positive control

HeLa cell extracts.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab53049 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

  • Western blot - Cullin 1 antibody (ab53049)Western blot - Cullin 1 antibody (ab53049) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab53049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

Target

Function

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

Tissue specificity

Expressed in lung fibroblasts.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the cullin family.

Post-translational
modifications

Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.

Target information above from: UniProt accessionQ13616 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CUL 1 antibody
  • CUL-1 antibody
  • CUL1 antibody
  • CUL1_HUMAN antibody
  • Cullin-1 antibody
  • Cullin1 antibody
  • MGC149834 antibody
  • MGC149835 antibody
see all

Database links

Anti-Cullin 1 antibody images:

  Western blot - Cullin 1 antibody (ab53049)

Western blot - Cullin 1 antibody (ab53049)

All lanes : Anti-Cullin 1 antibody (ab53049) at 1/500 dilution

Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunising peptide


Predicted band size : 90 kDa
Observed band size : 90 kDa

References for Anti-Cullin 1 antibody (ab53049)

ab53049 has not yet been referenced specifically in any publications.

Publishing research using ab53049? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"