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Western blot - Cullin 1 antibody (ab85152)
Western blot - Cullin 1 antibody (ab85152)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)
(enlarge)


Product Name 

Cullin 1 antibody

 

See all Cullin 1 antibodies (7)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Cullin 1

Immunogen 

A synthetic peptide from near the N terminal residues of human Cullin 1 (NM_003592).

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/50 - 1/200.
WB: 1/200 - 1/1000. Predicted molecular weight: 90 kDa. Peptide ELISA: 1/10000 - 1/40000.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate; colon cancer tissue.

Cellular localization 

Nuclear

Research areas 

Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

Relevance 

Cullin 1 may be involved in the degradation of positive cell cycle regulators (for example G1 cyclins in yeast). Human cullin 1 forms a complex with Skp1 and the F box protein Skp2, which is thought to play a role in the ubiquitination of G1 regulatory proteins, as its homolog does in yeast.

 

Alternative names Cullin 1 antibody (ab85152)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

8454    (Human)
26965    (Mouse)
362356    (Rat)

Omim

    

603134    (Human)

SwissProt

    

Q13616    (Human)
Q9WTX6    (Mouse)

Unigene

    

146806    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 2% BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cullin 1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Cullin 1 antibody images, references, products related to ab85152 and other tools.

 

Cullin 1 antibody images:

Product image  Western blot - Cullin 1 antibody (ab85152)

Western blot - Cullin 1 antibody (ab85152)

Cullin 1 antibody (ab85152) at 1/500 dilution + HeLa cell lysate

Predicted band size : 90 kDa
Observed band size : 90 kDa

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)

ab85152, at a 1/100 dilution, staining Cullin 1 in formalin fixed, paraffin embedded colon cancer tissue by Immunohistochemistry.

 
 

Search PubMed (MEDLINE) for references to Cullin 1

 

Cullin 1 antibody - more information

Customer reviews (feedback) regarding Cullin 1 antibody

Customer FAQs regarding Cullin 1 antibody

Protocols for Cullin 1 antibody

Price and availability of products related to Cullin 1 antibody

 

Cullin 1 antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Positive Controls for Cullin 1 antibody

ab14655

  

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

 

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Other Cullin 1 Antibodies

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Cullin 1 antibody - other tools:

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