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Anti-Cullin 1 antibody (ab85152)

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Overview

Product name

Anti-Cullin 1 antibody
See all Cullin 1 products (9) ...

Description

Rabbit polyclonal to Cullin 1

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide from near the N terminal residues of human Cullin 1 (NM_003592).

Positive control

HeLa cell lysate; colon cancer tissue.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 2% BSA

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Cullin 1 antibody (ab85152)Western blot - Cullin 1 antibody (ab85152) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab85152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: 1/50 - 1/200.
WB: 1/200 - 1/1000. Predicted molecular weight: 90 kDa. Peptide ELISA: 1/10000 - 1/40000.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

Tissue specificity

Expressed in lung fibroblasts.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the cullin family.

Post-translational
modifications

Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.

Target information above from: UniProt accessionQ13616 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CUL 1 antibody
  • CUL-1 antibody
  • CUL1 antibody
  • CUL1_HUMAN antibody
  • Cullin-1 antibody
  • Cullin1 antibody
  • MGC149834 antibody
  • MGC149835 antibody
see all

Anti-Cullin 1 antibody images:

  Western blot - Cullin 1 antibody (ab85152)

Western blot - Cullin 1 antibody (ab85152)

Anti-Cullin 1 antibody (ab85152) at 1/500 dilution + HeLa cell lysate

Predicted band size : 90 kDa
Observed band size : 90 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)

ab85152, at a 1/100 dilution, staining Cullin 1 in formalin fixed, paraffin embedded colon cancer tissue by Immunohistochemistry.

References for Anti-Cullin 1 antibody (ab85152)

ab85152 has not yet been referenced specifically in any publications.

Publishing research using ab85152? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"