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Western blot - Cullin 1 antibody (ab85152)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)
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Product Name
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Cullin 1 antibody
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See all Cullin 1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cullin 1
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Immunogen
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A synthetic peptide from near the N terminal residues of human Cullin 1 (NM_003592).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/50 - 1/200.
WB: 1/200 - 1/1000. Predicted molecular weight: 90 kDa. Peptide ELISA: 1/10000 - 1/40000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate; colon cancer tissue.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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Cullin 1 may be involved in the degradation of positive cell cycle regulators (for example G1 cyclins in yeast). Human cullin 1 forms a complex with Skp1 and the F box protein Skp2, which is thought to play a role in the ubiquitination of G1 regulatory proteins, as its homolog does in yeast.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 2% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cullin 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cullin 1 antibody images, references, products related to ab85152 and other tools.
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Cullin 1 antibody images:
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 Western blot - Cullin 1 antibody (ab85152)
Cullin 1 antibody (ab85152) at 1/500 dilution + HeLa cell lysate
Predicted band size : 90 kDa
Observed band size : 90 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)
ab85152, at a 1/100 dilution, staining Cullin 1 in formalin fixed, paraffin embedded colon cancer tissue by Immunohistochemistry.
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Search PubMed (MEDLINE) for references to Cullin 1
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Cullin 1 antibody - more information
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Customer reviews (feedback) regarding Cullin 1 antibody |
Customer FAQs regarding Cullin 1 antibody |
Protocols for Cullin 1 antibody |
Price and availability of products related to Cullin 1 antibody |
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Cullin 1 antibody - related products:
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Cullin 1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Cullin 1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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