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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-Cullin 1 antibody
See all Cullin 1 products (9) ...
Description
Rabbit polyclonal to Cullin 1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide from near the N terminal residues of human Cullin 1 (NM_003592).
Positive control
HeLa cell lysate; colon cancer tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 2% BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: 1/50 - 1/200.
WB: 1/200 - 1/1000. Predicted molecular weight: 90 kDa. Peptide ELISA: 1/10000 - 1/40000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
Tissue specificity
Expressed in lung fibroblasts.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the cullin family.
Post-translational
modifications
Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
Target information above from: UniProt accessionQ13616
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CUL 1 antibody
- CUL-1 antibody
- CUL1 antibody
see all
Database links
- Entrez Gene: 8454 Human
- Entrez Gene: 26965 Mouse
- Entrez Gene: 362356 Rat
- Omim: 603134 Human
- SwissProt: Q13616 Human
- SwissProt: Q9WTX6 Mouse
- Unigene: 146806 Human
- Unigene: 87611 Mouse
see all
Anti-Cullin 1 antibody images:
Western blot - Cullin 1 antibody (ab85152)
Anti-Cullin 1 antibody (ab85152) at 1/500 dilution + HeLa cell lysate
Predicted band size : 90 kDa
Observed band size : 90 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 1 antibody (ab85152)
ab85152, at a 1/100 dilution, staining Cullin 1 in formalin fixed, paraffin embedded colon cancer tissue by Immunohistochemistry.
References for Anti-Cullin 1 antibody (ab85152)
ab85152 has not yet been referenced specifically in any publications.
Publishing research using ab85152? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"