Overview
Product nameCullin 1 protein (Tagged-His Tag)See all Cullin 1 proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 525-776 (252 amino acids) of Human Cullin 1 with N terminal His tag; Predicted MWt 30 kDa (UniProt Q13616).
Expression hostE. coli
Properties
Purification notesPurity level >85%
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography.
In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag.
In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight.
This may be as a result of unequally distributed charges in the amino acid sequence.
Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
FormLyophilised:Reconstitute with 83 µl aqua dest.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Concentration information loading...
Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
Sequence notesLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQ RFTAFYASRHSG
RKLTWLYQLSKGELVTNCFKNRYTLQ ASTFQMAILLQYNTEDAYTVQQLTDS
TQIKMDILAQVL QILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKL RVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRI MKMRKVLKHQQLLG
EVLTQLSSRFKPRVPVIKKCIDIL IEKEYLERVDGEKDTYSYLA
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab91876
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
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| MS |
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Application notesMS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
CUL1_HUMANCullin-1Cullin1MGC149834MGC149835
see all
FunctionCore component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
Tissue specificityExpressed in lung fibroblasts.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the cullin family.
Post-translational
modificationsNeddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
Cullin 1 protein (Tagged-His Tag) images
References for Cullin 1 protein (Tagged-His Tag) (ab91876)
ab91876
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - Cullin 1 protein (Tagged-His Tag) (ab91876)The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.