Anti-Cullin 4A/CUL-4A antibody (ab72548)
Key features and details
- Rabbit polyclonal to Cullin 4A/CUL-4A
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Cullin 4A/CUL-4A antibody
See all Cullin 4A/CUL-4A primary antibodies -
Description
Rabbit polyclonal to Cullin 4A/CUL-4A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Chicken, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Sea urchin, Platypus -
Immunogen
Synthetic peptide corresponding to Human Cullin 4A/CUL-4A (C terminal).
Database link: NP_001008895.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
|
IP |
Use at 2-5 µg/mg of lysate.
|
Notes |
---|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
-
Function
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the cullin family. -
Post-translational
modificationsNeddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome. - Information by UniProt
-
Database links
- Entrez Gene: 8451 Human
- Entrez Gene: 99375 Mouse
- Omim: 603137 Human
- SwissProt: Q13619 Human
- SwissProt: Q3TCH7 Mouse
- Unigene: 339735 Human
- Unigene: 212861 Mouse
-
Alternative names
- 2810470J21Rik antibody
- AW495282 antibody
- CUL 4A antibody
see all
Images
-
All lanes : Anti-Cullin 4A/CUL-4A antibody (ab72548) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 88 kDa
Observed band size: 88 kDa
Additional bands at: 117 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Cullin 4A/CUL-4A with ab72548 at 1/1000 (1µg/ml). Detection: DAB.
-
Detection of Human Cullin 4A/CUL-4A by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72548 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
-
IHC image of ab72548 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72548, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (17)
ab72548 has been referenced in 17 publications.
- Kumar N et al. Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins. Nat Commun 13:974 (2022). PubMed: 35190564
- Lin H et al. IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion. Nat Commun 12:2461 (2021). PubMed: 33911083
- Townsend A et al. DCAF14 promotes stalled fork stability to maintain genome integrity. Cell Rep 34:108669 (2021). PubMed: 33503431
- Kim SA et al. Aryl Sulfonamides Induce Degradation of Aryl Hydrocarbon Receptor Nuclear Translocator through CRL4DCAF15 E3 Ligase. Mol Cells 43:935-944 (2020). PubMed: 33168788
- Nakade H et al. Overexpression of Cullin4A correlates with a poor prognosis and tumor progression in esophageal squamous cell carcinoma. Int J Clin Oncol 25:446-455 (2020). PubMed: 31535245