Overview
- Product nameAnti-CXCL14 antibodySee all CXCL14 primary antibodies ...
- DescriptionRabbit polyclonal to CXCL14
- Tested applicationsELISA, WB, ICC/IF, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
Highly pure (>98%) recombinant hBRAK (human BRAK).
- General notesThe sterile filtered antibody solution was lyophilized from 0.5xPBS, pH7.4.
Properties
- FormLyophilised:Reconstitute 100µl sterile water to give a concentration 1 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab46010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: Use a concentration of 0.5 µg/ml. Detection limit 0.2 - 0.4 ng/well. |
| WB | WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa. Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducting conditions. |
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
Target
- FunctionPotent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
- Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.
- Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
- Cellular localizationSecreted.
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Database links
- Entrez Gene: 9547 Human
- Omim: 604186 Human
- SwissProt: O95715 Human
- Unigene: 483444 Human
Target information above from: UniProt accession
O95715
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 1110031L23Rik antibody1200006I23Rik antibodyAI414372 antibody
- BMAC antibodybolekine antibodyBRAK antibodyC-X-C motif chemokine 14 antibodyChaemokine, CXC motif, ligand 14 antibodyChemokine (C-X-C motif) ligand 14 antibodyChemokine BRAK antibodyCXC chemokine in breast and kidney antibodyCxcl14 antibodyCXL14_HUMAN antibodyJSC antibodyKec antibodyKidney-expressed chemokine CXC antibodyKS1 antibodyMGC10687 antibodyMGC124510 antibodyMGC90667 antibodyMIP 2 gamma antibodyMIP-2G antibodyMIP2G antibodyMIP2gamma antibodyNJAC antibodyPRO273 antibodyPSEC0212 antibodyScyb14 antibodySmall Inducible Cytokine B14 antibodySmall inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK) antibodySmall Inducible Cytokine subfamily B, member 14 antibodySmall nducible cytokine B14 antibodySmall-inducible cytokine B14 antibodyUNQ240 antibody
see all
Anti-CXCL14 antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cxcl14/BRAK antibody (ab46010)ab46010 staining Cxcl14/BRAK in human breast invasive, ductal carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cxcl14/BRAK antibody (ab46010)ab46010 staining Cxcl14/BRAK in human breast invasive, ductal carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
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Immunocytochemistry/ Immunofluorescence-CXCL14 antibody(ab46010)ICC/IF image of ab46010 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46010 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-CXCL14 antibody (ab46010)
This product has been referenced in:
- Leach RE et al. High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples. Hum Reprod 27:814-828 (2012). IHC-P ; Human . Read more (PubMed: 22215622) »
- Gu XL et al. Expression of CXCL14 and its anticancer role in breast cancer. Breast Cancer Res Treat 135:725-35 (2012). WB . Read more (PubMed: 22910931) »
