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Overview

  • Product nameCyGEL Sustain™
  • Tested applicationsICC/IF, Fluorescence Microscopy, Functional Studies more details
  • General notesCyGEL Sustain™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL Sustain™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling. CyGEL Sustain™ has many applications in imaging and cell-based screening.
    Key features of CyGEL Sustain™ include:
    • Convenient immobilization of non-adherent cells.
    • Controllable and rapid transition from liquid to gel, which allows quick perparation of live cells for gel mounting.
    • Optically clear with low autofluorescence.
    • Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).

    CyGEL Sustain™ is recommended for mounting live de-adherent/ non-adherent cells and Zebrafish embryos for microscopy. CyGEL Sustain™ is specially formulated to allow addition of RPMI and similar culture media, which makes it the best tool for extended imaging experiments, as cells will survive for several hours.
    CyGEL Sustain™ is supplied as 10 vials, each containing 500µl.
    For live C. elegans and parasites we recommend CyGEL™(ab109204).

    • Properties

  • Storage instructionsStore at +4°C. Do not freeze.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab109205 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ICC/IF ICC/IF: Use at an assay dependent dilution.
    Please refer to the Protocol Booklet for detailed information about the use of CyGEL Sustain.
    Fluorescence Microscopy Fluorescence Microscopy: Use at an assay dependent concentration.
    Functional Studies FuncS: Use at an assay dependent dilution.

    CyGEL Sustain™ images

    • - CyGEL Sustain™ (ab109205)
      - CyGEL Sustain™ (ab109205)
      This ilustration demonstrates the general procedure for using CyGEL Sustain™ with live cells prior analysis by fluorescence microscopy.

    Protocols

    References for CyGEL Sustain™ (ab109205)

    ab109205 has not yet been referenced specifically in any publications.

    Publishing research using ab109205 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab109205 Submit a Question for ab109205

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

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    I am interested in using cygel (ab109204) for medium term (up to 20 hours) live imaging of insect embryos , and possibly as a way to immobilize them for injection. I saw that there have been sucessful uses of cygel for Drosophila, but only for shorter ter...

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    Thank you for contacting us.



    As suggested, there is no particular reason to use CyGEL Sustain over CyGEL (a PBS-based formulation) other than the ease of adding specific media.

    We have limited data on the long-term imagin...

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    Möchte ab109205 mindestens für 6 Stunden mit Insektenzellen benutzen. Länger ist besser. Was für Erfahrungswerte gibt es?

    Vielen Dank für Ihren Anruf. Ich habe nun eine Antwort aus dem Labor erhalten. Leider haben wir nur Erfahrung mit humanen Zellen für bis zu 4 Stunden in CyGEL. Es wird allerding dringend empfohlen das Gel mit Medium und anderen benötigten Z...

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    Dear ,

    I am interested to buy CyGel, I have trouble to enter in your web page. Can you send me please information about the product? I pretend to use it in Laser scanning confocal microscopy to work with cell culture at room temperature and wit...

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    Thank you for contacting us.

    I am sorry you are not able to enter our Website, please do let us know if the problem persists. You will find attached the protocols for our CyGel products: ab109205 and ab109204.

    These products should wor...

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    I am interested in performing a trial with CyGEL to immobilize suspension mammalian cells for time-lapse microscopy in a 96 well format. I did find the protocol PDF regarding the product but it only mentions that CuGEL can be used in a 96 well format. Do ...

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    Thank you for contacting Abcam.

    We do not have any data for this product being used in a 96-well format but that does not mean to say it will not work, just that we have not yet tested it in that format.

    I am sorry that I am not able t...

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    I received the wrong product (again), this time it was ab109204, instead of ab109205.

    As requested, I have issued a free of charge replacement for one kit of ab109205. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"