CyGEL Sustain™ (ab109205)
Key features of CyGEL Sustain™ include:
- Convenient immobilization of non-adherent cells.
- Controllable and rapid transition from liquid to gel, which allows quick perparation of live cells for gel mounting.
- Optically clear with low autofluorescence.
- Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).
CyGEL Sustain™ is recommended for mounting live de-adherent/ non-adherent cells and Zebrafish embryos for microscopy. CyGEL Sustain™ is specially formulated to allow addition of RPMI and similar culture media, which makes it the best tool for extended imaging experiments, as cells will survive for several hours.
CyGEL Sustain™ is supplied as 10 vials, each containing 500µl.
For live C. elegans and parasites we recommend CyGEL™(ab109204).
Our Abpromise guarantee covers the use of ab109205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent dilution. Please refer to the Protocol Booklet for detailed information about the use of CyGEL Sustain.|
|Fluorescence Microscopy||Fluorescence Microscopy: Use at an assay dependent concentration.|
|Functional Studies||FuncS: Use at an assay dependent dilution.|
CyGEL Sustain™ images
This ilustration demonstrates the general procedure for using CyGEL Sustain™ with live cells prior analysis by fluorescence microscopy.
References for CyGEL Sustain™ (ab109205)
ab109205 has not yet been referenced specifically in any publications.