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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab16726? Please let us know so that we can cite the reference in this datasheet
ab16726 has been referenced in 2 publications.
- McMains E et al. Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina. PLoS One 6:e17647 (2011). ICC/IF; Human. PubMed: 21408174
- Lyman SK et al. High-content, high-throughput analysis of cell cycle perturbations induced by the HSP90 inhibitor XL888. PLoS One 6:e17692 (2011). ICC/IF; Human. PubMed: 21408192
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody [6E6] (ab16726)](/ps/datasheet/Images/16/ab16726/ab16726_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody [6E6] (ab16726)
ab16726 staining human tonsil by IHC-P.
![Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [6E6] (ab16726)](/ps/datasheet/images/16/ab16726/Cyclin-A-Primary-antibodies-ab16726-1.jpg)
Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [6E6] (ab16726)
ICC/IF image of ab16726 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16726, 1/1000 dilution) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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