Anti-Cyclin A antibody [6E6] (ab16726)

Overview

• Product nameAnti-Cyclin A antibody [6E6]See all Cyclin A primary antibodies ...
• Description
  Mouse monoclonal [6E6] to Cyclin A
• Tested applicationsIHC-P, WB, IP, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide (Human) (N terminal).

• Positive controlTonsil

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA, Tissue culture supernatant
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone number6E6
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin A family

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipoproteins/Apolipoproteins
  • Apolipoproteins

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin A Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin A Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.

Target information above from: UniProt accession P20248 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 890 Human
  • Omim: 123835 Human
  • SwissProt: P20248 Human
  • Unigene: 58974 Human

• Alternative names
  CCN1 antibodyCCN1 antibodyCCNA antibody

  CCNA antibodyCCNA2 antibodyCCNA2_HUMAN antibodyCyclin A2 antibodyCyclin A2 antibodyCyclin A2 antibodyCyclin-A antibodyCyclin-A2 antibody

  see all

Anti-Cyclin A antibody [6E6] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody [6E6] (ab16726)
  ab16726 staining human tonsil by IHC-P.
• 

  Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [6E6] (ab16726)
  ICC/IF image of ab16726 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16726, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.