![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody [6E6] (ab16726) image](#ab16726_1.jpg)
![Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [6E6] (ab16726) image](#Cyclin-A-Primary-antibodies-ab16726-1.jpg)
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Products:Cell Biology >> Cell Cycle >> Cyclins
Overview
Product name
Anti-Cyclin A antibody [6E6]
See all Cyclin A products (20) ...
Description
Mouse monoclonal [6E6] to Cyclin A
Tested applications
IHC-P, WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) (N terminal).
Positive control
Tonsil
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
6E6
Isotype
IgG1
Light chain type
kappa
Research areas
Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Apolipoproteins
Cancer >> Cell cycle >> Cyclins >> Cyclin A family
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/20 - 1/40.
WB: Use at an assay dependent dilution. (1/100 - 1/500.)
IP: Use at an assay dependent dilution. (1/100 - 1/500.)
ICC/IF: 1/1000
Target
Function
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily.
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
Target information above from: UniProt accessionP20248
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCN1 antibody
- CCN1 antibody
- CCNA antibody
see all
Database links
- Entrez Gene: 890 Human
- Omim: 123835 Human
- SwissProt: P20248 Human
- Unigene: 58974 Human
Anti-Cyclin A antibody [6E6] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody [6E6] (ab16726)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A antibody [6E6] (ab16726)](/ps/datasheet/Images/16/ab16726/ab16726_1.jpg)
ab16726 staining human tonsil by IHC-P.
Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [6E6] (ab16726)
![Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [6E6] (ab16726)](/ps/datasheet/images/16/ab16726/Cyclin-A-Primary-antibodies-ab16726-1.jpg)
ICC/IF image of ab16726 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16726, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Cyclin A antibody [6E6] (ab16726)
This product has been referenced in:
- McMains Eet al. Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina. PLoS One 6:e17647 (2011). ICC/IF; Human.Read more (PubMed: 21408174) »
- Lyman SKet al. High-content, high-throughput analysis of cell cycle perturbations induced by the HSP90 inhibitor XL888. PLoS One 6:e17692 (2011). ICC/IF; Human.Read more (PubMed: 21408192) »
See all 2 publications for this product
Publishing research using ab16726? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"