Anti-Cyclin A antibody [6E6], prediluted (ab74444)

Overview

  • Product nameAnti-Cyclin A antibody [6E6], predilutedSee all Cyclin A2 primary antibodies ...
  • Description
    Mouse monoclonal [6E6] to Cyclin A, prediluted
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant N-terminal fragment of human Cyclin A protein.

  • EpitopeN-terminal
  • Positive control
    • Human Tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab74444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
  • Application notesIHC-P: Ready to use. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Target information above from: UniProt accession P20248 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CCN1 antibody
      • CCNA antibody
      • CCNA2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Anti-Cyclin A antibody [6E6], prediluted images

    • Formalin-fixed, paraffin-embedded human tonsil stained with ab74444 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.

    References for Anti-Cyclin A antibody [6E6], prediluted (ab74444)

    ab74444 has not yet been referenced specifically in any publications.

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