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Western blot - Cyclin A antibody [E23.1] (ab38)
Western blot - Cyclin A antibody [E23.1] (ab38)
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Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E23.1] (ab38)
Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E23.1] (ab38)
(enlarge)


Immunoprecipitation - Cyclin A antibody [E23.1] (ab38)
Immunoprecipitation - Cyclin A antibody [E23.1] (ab38)
(enlarge)


Product Name 

Cyclin A antibody [E23.1]

 

See all Cyclin A antibodies (18)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [E23.1] to Cyclin A

Immunogen 

Full length protein (Cow).

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Cow

Tested applications 
(see key)

ICC/IF, IHC-FoFr, IHC-Fr, IP, WB


Abreviews 

Customer reviews
(see key)


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• IP

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• WB

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IP
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WB
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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 19429027).
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Recombinant cyclin A

Cellular localization 

Nuclear

Research areas 

Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Apolipoproteins
Cancer >> Cell cycle >> Cyclins >> Cyclin A family
Chromatin and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin A Family

Relevance 

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.

 

Alternative names Cyclin A antibody [E23.1] (ab38)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

281667    (Cow)
890    (Human)
12428    (Mouse)
114494    (Rat)

Omim

    

123835    (Human)

SwissProt

    

P20248    (Human)
P51943    (Mouse)

Unigene

    

58974    (Human)
13094    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

E23.1

Myeloma 

Sp2

Isotype 

IgG2a

Purity 

Protein A purified

Storage buffer 

Phosphate buffered saline

Form 

Liquid

Concentration 

1.200 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cyclin A antibody [E23.1] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Cyclin A antibody [E23.1] images, references, products related to ab38 and other tools.

 

Cyclin A antibody [E23.1] images:

Product image  Western blot - Cyclin A antibody [E23.1] (ab38)

Western blot - Cyclin A antibody [E23.1] (ab38)



Predicted band size : 60 kDa


Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.

Julian Gannon, CRUK.

 

Product image  Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E23.1] (ab38)

Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E23.1] (ab38)

ICC/IF image of ab38 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab38, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

 

Product image  Immunoprecipitation - Cyclin A antibody [E23.1] (ab38)

Immunoprecipitation - Cyclin A antibody [E23.1] (ab38)

Immunoprecipitation using ab38 at 4µg/mg whole cell lysate diluted in RIPA buffer and incubated with Protein G matrix at 4°C for 12 hours.

This antibody can immunoprecipitate Cyclin A from both Human (293T) and Rat (PC12) cell extract. The species assigned as Cyclin A runs at  ~55 kDa.

Lanes 1-3 = 293T

Lanes 4-6 = PC12

Lane 1: Cyclin A antibody IP

Lane 2: Control antibody IP

Lane 3: 293T whole cell lysate at 10%

Lane 4: Cyclin A antibody IP

Lane 5: Control antibody IP

Lane 6: PC12 whole cell lysate at 10%

The Western Blot antibody was ab38 at 2µg/ml

This image is courtesy of an anonymous Abreview

See Abreview

 

Product image  Western blot - Cyclin A antibody [E23.1] (ab38)

Western blot - Cyclin A antibody [E23.1] (ab38)

All lanes : Cyclin A antibody [E23.1] (ab38) at 2 µg/ml

Lane 1 : U2OS (Hu) whole cell lysate
Lane 2 : 293T (Hu) whole cell lysate
Lane 3 : Cos-7 (AGMk) whole cell lysate
Lane 4 : CHO (Hm) whole cell lysate
Lane 5 : PC12 (Rat) whole cell lysate
Lane 6 : 3T3 (Ms) whole cell lysate

Lysates/proteins at 30 µg per lane.

Secondary
HRP conjugated goat anti-mouse at 1/5000 dilution

Performed under reducing conditions.

Predicted band size : 60 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 6 minutes

The membrane was blocked with 5% milk for 1 hour at 21°C prior to incubation with the primary antibody for 12 hours at 4°C.

This image is courtesy of an anonymous Abreview

See Abreview

 

References for Cyclin A antibody [E23.1] (ab38)

Specific publication references   This product has been used in: (two most recent references)

Bourke E et al. DNA damage induces Chk1-dependent threonine-160 phosphorylation and activation of Cdk2. Oncogene 29:616-24 (2010). WB; Chicken. PubMed: 19838212

Chen J et al. Decreased level of cyclin A2 in rat cochlea development and cochlear stem cell differentiation. Neurosci Lett 453:166-9 (2009). WB, ICC/IF, IHC-FoFr; Rat. PubMed: 19429027


See all 4 publication references for this product.


If you publish research using ab38 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Cyclin A

 

Cyclin A antibody [E23.1] - more information

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Cyclin A antibody [E23.1] - related products:

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