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Overview

  • Product nameAnti-Cyclin A antibody [E23.1]See all Cyclin A primary antibodies ...
  • Description
    Mouse monoclonal [E23.1] to Cyclin A
  • Tested applicationsICC, IHC-P, IHC-FoFr, WB, IP, IHC-Fr, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Full length protein (Cow).

  • Positive controlWB: Recombinant cyclin A This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab38 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC ICC: Use at an assay dependent concentration.
IHC-P IHC-P: Use a concentration of 5 µg/ml.
IHC-FoFr IHC-FoFr: Use at an assay dependent dilution.
WB WB: Use a concentration of 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP IP: Use at an assay dependent dilution.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.
Flow Cyt Flow Cyt: Use at an assay dependent concentration. PubMed: 22802651
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.

Target

  • Alternative names
      CCN1 antibodyCCNA antibodyCcna2 antibody
      CCNA2_HUMAN antibodyCyclin A2 antibodyCyclin-A antibodyCyclin-A2 antibody
    see all

Anti-Cyclin A antibody [E23.1] images



  • Predicted band size : 60 kDa


    Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.

    Julian Gannon, CRUK.

  • ICC/IF image of ab38 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab38, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Immunoprecipitation using ab38 at 4µg/mg whole cell lysate diluted in RIPA buffer and incubated with Protein G matrix at 4°C for 12 hours.

    This antibody can immunoprecipitate Cyclin A from both Human (293T) and Rat (PC12) cell extract. The species assigned as Cyclin A runs at  ~55 kDa.

    Lanes 1-3 = 293T

    Lanes 4-6 = PC12

    Lane 1: Cyclin A antibody IP

    Lane 2: Control antibody IP

    Lane 3: 293T whole cell lysate at 10%

    Lane 4: Cyclin A antibody IP

    Lane 5: Control antibody IP

    Lane 6: PC12 whole cell lysate at 10%

    The Western Blot antibody was ab38 at 2µg/ml

    See Abreview

  • All lanes : Anti-Cyclin A antibody [E23.1] (ab38) at 2 µg/ml

    Lane 1 : U2OS (Hu) whole cell lysate
    Lane 2 : 293T (Hu) whole cell lysate
    Lane 3 : Cos-7 (AGMk) whole cell lysate
    Lane 4 : CHO (Hm) whole cell lysate
    Lane 5 : PC12 (Rat) whole cell lysate
    Lane 6 : 3T3 (Ms) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP conjugated goat anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Cyclin A antibody [E23.1] (ab38)

This product has been referenced in:
  • Li VC  et al. Gap 1 phase length and mouse embryonic stem cell self-renewal. Proc Natl Acad Sci U S A 109:12550-5 (2012). Flow Cyt . Read more (PubMed: 22802651) »
  • He Z  et al. Optimization of immunolocalization of cell cycle proteins in human corneal endothelial cells. Mol Vis 17:3494-511 (2011). IHC - Wholemount ; Human . Read more (PubMed: 22219645) »

See all 9 Publications for this product

Product Wall

Displaying 1 - 10 of 12 results for Abreviews and Q&A



The non hazardous SDS forms have been placed as 'SDS' PDF files available for download from the online product datasheets for ab9574 and ab87352 on Abcam's website.

Alternatively I have placed links to the PDF files below:

SD...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (corneal endothelium)
Specification corneal endothelium
Fixative Formaldehyde
Permeabilization Yes - 1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Verified customer

Submitted Nov 06 2012

Application Immunocytochemistry
Sample Human Cultured Cells (corneal endothelial cells in vitro)
Specification corneal endothelial cells in vitro
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Verified customer

Submitted Nov 06 2012

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one o...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital inf...

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Thank you very much for confirming the details.

We are unsure about the identity of bands. Not many complaints are received for this antibody infact this is the second since December 2011 so protocol troubleshooting might help.

There is ...

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Thanks you very much for your email. I am very sorry to hear that you are experiencing problems with this antibody.

I have checked the image and details you have kindly provided and in order to know more about the problem I have following questio...

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293T)
Total protein in input 200 µg
Specification 293T
Immuno-precipitation step Protein G
Username

Verified customer

Submitted Nov 13 2007

Application Western blot
Sample Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount 30 µg
Specification U2OS and 293T cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Verified customer

Submitted Nov 07 2007

I'm very sorry to hear you are having problems with ab38. We do not have a recommended starting dilution so I would like to recommend trying a higher concentration of antibody (try 10ug/ml). We have only received one complaint regarding this popular a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"