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Western blot - Cyclin A antibody [E23.1] (ab38)
![Western blot - Cyclin A antibody [E23.1] (ab38)](/ps/datasheet/images/ab38_1.jpg)
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Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E23.1] (ab38)
![Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E23.1] (ab38)](/ps/datasheet/images/ab38-OEM-060623-Im1.jpg)
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Immunoprecipitation - Cyclin A antibody [E23.1] (ab38)
![Immunoprecipitation - Cyclin A antibody [E23.1] (ab38)](/ps/datasheet/images/ab38_2.jpg)
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Product Name
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Cyclin A antibody [E23.1]
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See all Cyclin A antibodies (18)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [E23.1] to Cyclin A
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Immunogen
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Full length protein (Cow).
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Cow
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 19429027).
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Recombinant cyclin A
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Cellular localization
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Nuclear
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Research areas
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Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Apolipoproteins
Cancer >> Cell cycle >> Cyclins >> Cyclin A family
Chromatin and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin A Family
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Relevance
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Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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E23.1
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Myeloma
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Sp2
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Isotype
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IgG2a
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Purity
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Protein A purified
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Storage buffer
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Phosphate buffered saline
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Form
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Liquid
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Concentration
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1.200 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cyclin A antibody [E23.1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cyclin A antibody [E23.1] images, references, products related to ab38 and other tools.
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Cyclin A antibody [E23.1] images:
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 Western blot - Cyclin A antibody [E23.1] (ab38)
Predicted band size : 60 kDa
Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.
Julian Gannon, CRUK.
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Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E23.1] (ab38)
ICC/IF image of ab38 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab38, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Immunoprecipitation - Cyclin A antibody [E23.1] (ab38)
Immunoprecipitation using ab38 at 4µg/mg whole cell lysate diluted in RIPA buffer and incubated with Protein G matrix at 4°C for 12 hours. This antibody can immunoprecipitate Cyclin A from both Human (293T) and Rat (PC12) cell extract. The species assigned as Cyclin A runs at ~55 kDa. Lanes 1-3 = 293T Lanes 4-6 = PC12 Lane 1: Cyclin A antibody IP Lane 2: Control antibody IP Lane 3: 293T whole cell lysate at 10% Lane 4: Cyclin A antibody IP Lane 5: Control antibody IP Lane 6: PC12 whole cell lysate at 10% The Western Blot antibody was ab38 at 2µg/ml This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - Cyclin A antibody [E23.1] (ab38)
![Western blot - Cyclin A antibody [E23.1] (ab38)](/ps/datasheet/images/ab38_3.jpg)
All lanes : Cyclin A antibody [E23.1] (ab38) at 2 µg/ml
Lane 1 : U2OS (Hu) whole cell lysate
Lane 2 : 293T (Hu) whole cell lysate
Lane 3 : Cos-7 (AGMk) whole cell lysate
Lane 4 : CHO (Hm) whole cell lysate
Lane 5 : PC12 (Rat) whole cell lysate
Lane 6 : 3T3 (Ms) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
HRP conjugated goat anti-mouse at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 6 minutes
The membrane was blocked with 5% milk for 1 hour at 21°C prior to incubation with the primary antibody for 12 hours at 4°C. This image is courtesy of an anonymous Abreview
See Abreview
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References for Cyclin A antibody [E23.1] (ab38)
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This product has been used in: (two most recent references)
Bourke E et al. DNA damage induces Chk1-dependent threonine-160 phosphorylation and activation of Cdk2. Oncogene 29:616-24 (2010). WB; Chicken. PubMed: 19838212
Chen J et al. Decreased level of cyclin A2 in rat cochlea development and cochlear stem cell differentiation. Neurosci Lett 453:166-9 (2009). WB, ICC/IF, IHC-FoFr; Rat. PubMed: 19429027
See all 4 publication references for this product.
If you publish research using ab38 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cyclin A
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Cyclin A antibody [E23.1] - more information
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Cyclin A antibody [E23.1] - related products:
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Cyclin A antibody [E23.1] - other tools:
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Contact Abcam with a Technical Enquiry about Cyclin A antibody [E23.1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Cyclin A antibody [E23.1] (ab38)](/index.html?datasheet=38#ab38_1.jpg){kind=small}
![Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E23.1] (ab38)](/index.html?datasheet=38#ab38-OEM-060623-Im1.jpg)
![Immunoprecipitation - Cyclin A antibody [E23.1] (ab38)](/index.html?datasheet=38#ab38_2.jpg)
