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Anti-Cyclin A antibody [E67.1] (ab39)

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Overview

Product name

Anti-Cyclin A antibody [E67.1]
See all Cyclin A products (20) ...

Description

Mouse monoclonal [E67.1] to Cyclin A

Tested applications

ICC/IF, WB, ICC, IP, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Cow, Human

Immunogen

Full length protein (Cow).

Epitope

The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1

General notes

Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

E67.1

Myeloma

unknown

Isotype

IgG2a

Light chain type

unknown

Research areas

Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Apolipoproteins
Cancer >> Cell cycle >> Cyclins >> Cyclin A family
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins

  • Immunoprecipitation - Cyclin A antibody [E67.1] (ab39)Immunoprecipitation - Cyclin A antibody [E67.1] (ab39) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E67.1] (ab39)Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E67.1] (ab39) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab39 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.

Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Developmental stage

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

Cellular localization

Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.

Target information above from: UniProt accessionP20248 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CCN1 antibody
  • CCNA antibody
  • CCNA antibody
  • CCNA2 antibody
  • CCNA2_HUMAN antibody
  • Cyclin A2 antibody
  • Cyclin A2 antibody
  • Cyclin A2 antibody
  • Cyclin-A antibody
  • Cyclin-A2 antibody
see all

Database links

Anti-Cyclin A antibody [E67.1] images:

  Immunoprecipitation - Cyclin A antibody [E67.1] (ab39)

Immunoprecipitation - Cyclin A antibody [E67.1] (ab39)

Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.

  Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E67.1] (ab39)

Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E67.1] (ab39)

ab39 at a 1/100 dilution staining Cyclin A in African Green Monkey COS-7 cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in methanol and an Alexa-Fluor 555 conjugated anti-mouse secondary was used at a 1/3000 dilution.

Image courtesy of an anonymous Abreview.

References for Anti-Cyclin A antibody [E67.1] (ab39)

This product has been referenced in:

  • Ge XQ & Blow JJ Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories. J Cell Biol 191:1285-97 (2010). IP; Human.Read more (PubMed: 21173116) »
  • Ho KKet al. Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network. J Biol Chem 283:5477-85 (2008). ICC/IF; Mouse.Read more (PubMed: 18077453) »

See all 2 publications for this product

Publishing research using ab39? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"