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Read our guarantee »Products:Cell Biology >> Cell Cycle >> Cyclins
Anti-Cyclin A antibody [E67.1]
See all Cyclin A products (20) ...
Mouse monoclonal [E67.1] to Cyclin A
ICC/IF, WB, ICC, IP, IHC-Pmore details
Reacts with
Mouse, Cow, Human
Full length protein (Cow).
The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: PBS
Concentration information loading...
Protein A purified
Monoclonal
E67.1
unknown
IgG2a
unknown
Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Apolipoproteins
Cancer >> Cell cycle >> Cyclins >> Cyclin A family
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin A Family
Cell Biology >> Cell Cycle >> Cyclins
Our Abpromise guarantee covers the use of ab39 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 18077453)
WB: Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.
ICC: Use at an assay dependent dilution.
IP: Use a concentration of 2.5 µg/ml
IHC-P: Use at an assay dependent dilution.
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Belongs to the cyclin family. Cyclin AB subfamily.
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
Target information above from: UniProt accessionP20248
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunoprecipitation - Cyclin A antibody [E67.1] (ab39)
![Immunoprecipitation - Cyclin A antibody [E67.1] (ab39)](/ps/datasheet/Images/0/ab39/ab39_1.jpg)
Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.
Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E67.1] (ab39)
![Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E67.1] (ab39)](/ps/datasheet/images/0/ab39/Cyclin-A-Primary-antibodies-ab39-2.jpg)
ab39 at a 1/100 dilution staining Cyclin A in African Green Monkey COS-7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol and an Alexa-Fluor 555 conjugated anti-mouse secondary was used at a 1/3000 dilution.
Image courtesy of an anonymous Abreview.
This product has been referenced in:
See all 2 publications for this product
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![Immunoprecipitation - Cyclin A antibody [E67.1] (ab39)](/ps/datasheet/Images/0/ab39/ab39_1.jpg)
Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.
![Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E67.1] (ab39)](/ps/datasheet/images/0/ab39/Cyclin-A-Primary-antibodies-ab39-2.jpg)
ab39 at a 1/100 dilution staining Cyclin A in African Green Monkey COS-7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol and an Alexa-Fluor 555 conjugated anti-mouse secondary was used at a 1/3000 dilution.
Image courtesy of an anonymous Abreview.
2
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