Anti-Cyclin A antibody [E67.1] (ab39)

Overview

• Product nameAnti-Cyclin A antibody [E67.1]See all Cyclin A primary antibodies ...
• Description
  Mouse monoclonal [E67.1] to Cyclin A
• Tested applicationsICC/IF, WB, ICC, IP, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Cow, Human, African Green Monkey
  
• Immunogen

  Full length protein (Cow).

• EpitopeThe epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1
• General notesFew other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberE67.1
• Myelomaunknown
• IsotypeIgG2a
• Light chain typeunknown
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin A family

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipoproteins/Apolipoproteins
  • Apolipoproteins

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin A Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin A Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.

Target information above from: UniProt accession P20248 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 281667 Cow
  • Entrez Gene: 890 Human
  • Entrez Gene: 12428 Mouse
  • Omim: 123835 Human
  • SwissProt: P20248 Human
  • SwissProt: P51943 Mouse
  • Unigene: 58974 Human
  • Unigene: 4189 Mouse

• Alternative names
  CCN1 antibodyCCNA antibodyCCNA antibody

  CCNA2 antibodyCCNA2_HUMAN antibodyCyclin A2 antibodyCyclin A2 antibodyCyclin A2 antibodyCyclin-A antibodyCyclin-A2 antibody

  see all

Anti-Cyclin A antibody [E67.1] images

• 

  Immunoprecipitation - Cyclin A antibody [E67.1] (ab39)
  Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.
• 

  Immunocytochemistry/ Immunofluorescence - Cyclin A antibody [E67.1] (ab39)Image courtesy of an anonymous Abreview.
  ab39 at a 1/100 dilution staining Cyclin A in African Green Monkey COS-7 cells by Immunocytochemistry/ Immunofluorescence.
  
  Cells were fixed in methanol and an Alexa-Fluor 555 conjugated anti-mouse secondary was used at a 1/3000 dilution.