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Overview

  • Product nameAnti-Cyclin A antibody [Y193]See all Cyclin A primary antibodies ...
  • Description
    Rabbit monoclonal [Y193] to Cyclin A
  • Tested applicationsIHC-P, ICC, IP, WB more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of human Cyclin A.

  • Positive controlWB: HeLa cell lysate. IHC-P: Skin carcinoma.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab32386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
ICC
IP
WB
  • Application notesICC: 1/100 - 1/250.
    IHC-P: 1/50 - 1/100.
    IP: 1/50.
    WB: 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

    Is unsuitable for FACS.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.

      • Target information above from: UniProt accession P20248 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CCN1 antibodyCCNA antibodyCCNA2 antibody
          CCNA2_HUMAN antibodyCyclin A2 antibodyCyclin-A antibodyCyclin-A2 antibody
        see all

      Anti-Cyclin A antibody [Y193] images

      • Western blot - Cyclin A antibody [Y193] (ab32386)
        Western blot - Cyclin A antibody [Y193] (ab32386)
        Anti-Cyclin A antibody [Y193] (ab32386) at 1/1000 dilution + HeLa cell lysate

        Predicted band size : 48 kDa
        Observed band size : 55 kDa (why is the actual band size different from the predicted?)
      • Immunohistochemistry (Paraffin-embedded sections) - Cyclin A antibody [Y193] (ab32386)
        Immunohistochemistry (Paraffin-embedded sections) - Cyclin A antibody [Y193] (ab32386)
        Ab32386, at a 1/50 dilution, staining Cyclin A in paraffin embedded skin carcinoma tissue sections by Immunohistochemistry.

      References for Anti-Cyclin A antibody [Y193] (ab32386)

      This product has been referenced in:

      See 1 Publication for this product

      Publishing research using ab32386 ? Please let us know so that we can cite the reference in this datasheet

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