Overview

  • Product nameAnti-Cyclin A antibody [Y193]See all Cyclin A2 primary antibodies ...
  • Description
    Rabbit monoclonal [Y193] to Cyclin A
  • Tested applicationsIHC-P, ICC, IP, WB more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of human Cyclin A.

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Skin carcinoma.
  • General notes

    Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
ICC
IP
WB
  • Application notesICC: 1/100 - 1/250.
    IHC-P: 1/50 - 1/100.
    IP: 1/50.
    WB: 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

    Is unsuitable for FACS.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Target information above from: UniProt accession P20248 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CCN1 antibody
      • CCNA antibody
      • CCNA2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Anti-Cyclin A antibody [Y193] images

    • Anti-Cyclin A antibody [Y193] (ab32386) at 1/1000 dilution + HeLa cell lysate

      Predicted band size : 48 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • Ab32386, at a 1/50 dilution, staining Cyclin A in paraffin embedded skin carcinoma tissue sections by Immunohistochemistry.

    References for Anti-Cyclin A antibody [Y193] (ab32386)

    This product has been referenced in:

    See 1 Publication for this product

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