Anti-Cyclin A antibody [Y193] (ab32386)
- Product nameAnti-Cyclin A antibody [Y193]See all Cyclin A primary antibodies ...
- DescriptionRabbit monoclonal [Y193] to Cyclin A
- Tested applicationsIHC-P, ICC, IP, WB more details
- Species reactivityReacts with: Human
Does not react withMouse, Rat
Synthetic peptide corresponding to residues in the C terminus of human Cyclin A.
- Positive controlWB: HeLa cell lysate. IHC-P: Skin carcinoma.
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- Concentration information loading...
- Clonality Monoclonal
- Clone numberY193
- Research Areas
Our Abpromise guarantee covers the use of ab32386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
- Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
- Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
- Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
- CCN1 antibodyCCNA antibodyCCNA2 antibody
- CCNA2_HUMAN antibodyCyclin A2 antibodyCyclin-A antibodyCyclin-A2 antibody
Anti-Cyclin A antibody [Y193] images
Anti-Cyclin A antibody [Y193] (ab32386) at 1/1000 dilution + HeLa cell lysate
Predicted band size : 48 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Ab32386, at a 1/50 dilution, staining Cyclin A in paraffin embedded skin carcinoma tissue sections by Immunohistochemistry.