Overview

  • Product nameAnti-Cyclin A antibodySee all Cyclin A2 primary antibodies ...
  • Description
    Rabbit polyclonal to Cyclin A
  • SpecificityAntiserum will specifically react with a 60 kDa Cyclin A protein. No reaction was observed against other related cyclins.
  • Tested applicationsWB, IP, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Fusion protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab2097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
ELISA
ICC/IF
  • Application notesELISA: 1/500 - 1/2000.
    ICC/IF: Use at an assay dependent dilution (PMID 18083977).
    IP: Use at an assay dependent dilution.
    WB: 1/250 - 1/500. Predicted molecular weight: 49 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Cyclin A antibody images

    • ab2097, staining Cyclin A in rabbit vascular smooth muscle cells, by Immunocytochemistry/ Immunofluorescence.

      Cells were either untreated (left) or treated with 10 µM ceramide (right), fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated in blocking buffer (PBS, 2% skim milk, and 0.1% Tween-20) for 30 min at room temperature. Samples were incubated with primary antibody at 1/50 dilution for 1 hour at room temperature. An AlexaFluor®-conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.

    References for Anti-Cyclin A antibody (ab2097)

    This product has been referenced in:
    • Faustino RS  et al. Ceramide regulation of nuclear protein import. J Lipid Res 49:654-62 (2008). ICC/IF ; Rabbit . Read more (PubMed: 18083977) »
    • Rao HV  et al. Cyclin D1, cdk4, and Bim are involved in thrombin-induced apoptosis in cultured cortical neurons. J Neurochem 101:498-505 (2007). WB ; Rat . Read more (PubMed: 17254021) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry and your interest. The antibody you mentioned is a polyclonal antibody. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. Therefore, for polyclonal antibodies no ...

    Read More

    This antibody has only been tested on mammalian sources. It cross-reacts with rat and mouse. Presumably this molecule is so fundamental, that it is highly conserved in nature, but it hasn't been tested.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"