Anti-Cyclin A antibody (ab87359)

Overview

• Product nameAnti-Cyclin A antibodySee all Cyclin A primary antibodies ...
• Description
  Rabbit polyclonal to Cyclin A
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Hamster
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse Cyclin A.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab95425.)

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: F9; MEF1; K562.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin A family

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipoproteins/Apolipoproteins
  • Apolipoproteins

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin A Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin A Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.

Target information above from: UniProt accession P20248 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 890 Human
  • Entrez Gene: 12428 Mouse
  • Entrez Gene: 114494 Rat
  • Omim: 123835 Human
  • SwissProt: P20248 Human
  • SwissProt: P51943 Mouse
  • Unigene: 58974 Human
  • Unigene: 4189 Mouse

  • Unigene: 13094 Rat

  see all

• Alternative names
  CCN1 antibodyCCNA antibodyCCNA2 antibody

  CCNA2_HUMAN antibodyCyclin A2 antibodyCyclin-A antibodyCyclin-A2 antibody

  see all

Anti-Cyclin A antibody images

• 

  Western blot - Cyclin A antibody (ab87359)
  All lanes : Anti-Cyclin A antibody (ab87359) at 1 µg/ml
  
  Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
  Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 47 kDa
  Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 20 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Cyclin A antibody (ab87359)
  ICC/IF image of ab87359 stained HepG2 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87359, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A antibody (ab87359)
  ab87359 staining cyclin A in DU145 cells treated with lovastatin (ab120614), by ICC/IF. Decrease in cyclin A expression correlates with increased concentration of lovastatin, as described in literature.
  The cells were incubated at 37°C for 24h in media containing different concentrations of ab120614 (lovastatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab87359(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.