Anti-Cyclin A1 antibody (ab53699)

Overview

• Product nameAnti-Cyclin A1 antibodySee all Cyclin A1 primary antibodies ...
• Description
  Rabbit polyclonal to Cyclin A1
• SpecificityDetects endogenous levels of total Cyclin A1 protein.
• Tested applicationsICC/IF, WB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic peptide (Human)

• Positive controlSKOV3 cell extracts.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin A family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin A Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin A Family

Applications

Target

• FunctionMay be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
• Tissue specificityVery high levels in testis and very low levels in brain. Also found in myeloid Leukemia cell lines.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageExpression increases in early G1 phase and reaches highest levels during the S and G2/M phases.
• Cellular localizationNucleus.

Target information above from: UniProt accession P78396 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Clone ID: BC036346 Human
  • Entrez Gene: 8900 Human
  • Entrez Gene: 12427 Mouse
  • IMAGE Id: 5172478 Human
  • Omim: 604036 Human
  • SwissProt: P78396 Human
  • SwissProt: Q61456 Mouse
  • Unigene: 417050 Human

  • Unigene: 4815 Mouse

  see all

• Alternative names
  CCN A1 antibodyCCNA 1 antibodyCCNA1 antibody

  CCNA1_HUMAN antibodyCyclin-A1 antibodyCyclinA1 antibodyG2/mitotic specific cyclin A1 antibodyMGC132235 antibodyMGC159139 antibody

  see all

Anti-Cyclin A1 antibody images

• 

  Western blot - Cyclin A1 antibody (ab53699)
  All lanes : Anti-Cyclin A1 antibody (ab53699) at 1/500 dilution
  
  Lane 1 : SKOV3 cell extract
  Lane 2 : SKOV3 cell extract with immunising peptide
  
  
  Predicted band size : 52 kDa
  Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence-Cyclin A1 antibody(ab53699)
  ICC/IF image of ab53699 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53699, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin A1 antibody (ab53699)
  IHC image of ab53699 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53699, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.