Overview

  • Product nameAnti-Cyclin A2 antibody [E399]
    See all Cyclin A2 primary antibodies
  • Description
    Rabbit monoclonal [E399] to Cyclin A2
  • Specificityab32498 recognises Cyclin A2.
  • Tested applicationsWB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cyclin A2 aa 100-200 (N terminal).

  • Epitopeab32498 reacts with an epitope located in the N terminal region of Cyclin A2
  • Positive control
    • HeLa cells
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IP
  • Application notesICC/IF: 1/250 - 1/500.
    IP: 1/60.
    WB: 1/500. Predicted molecular weight: 48 kDa.
    Is unsuitable for FACS or IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCN1 antibody
      • CCNA antibody
      • CCNA2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Anti-Cyclin A2 antibody [E399] images

    • Anti-Cyclin A2 antibody [E399] (ab32498) at 1/500 dilution + HeLa cell lysate

      Predicted band size : 48 kDa
      Observed band size : 48 kDa
    • Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.

    References for Anti-Cyclin A2 antibody [E399] (ab32498)

    This product has been referenced in:
    • Yajima H  et al. The complexity of DNA double strand breaks is a critical factor enhancing end-resection. DNA Repair (Amst) 12:936-46 (2013). Read more (PubMed: 24041488) »
    • Edel MJ  et al. A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells. Cell Commun Signal 9:8 (2011). WB . Read more (PubMed: 21481269) »

    See all 2 Publications for this product

    Product Wall

    Ich denke wirklich, dass Ihr Problem mit diesen Aks nicht auf den WB begrenzt ist , da mir die ICC Färbung nicht spezifisch erscheint. Ich würde die Färbung definitiv eher nuklear lokalisiert erwarten, und das ist sie einfach nicht.
    Wir verwenden ...

    Read More


    Ich habe Ihnen einen Blot mit dem ab32498 an diese Email gehängt: von einem Schmier keine Spur! Was mir allerdings aufgefallen ist, ist dass der Verdünnungsfaktor um 10 verkleinert wurde. Ich habe unser Datenblatt dementsprechend verändert. Falls ...

    Read More

    Thank you for your inquiry. The lab has not tested this antibody for its cross-reactivity with Cyclin A1. But when we BLASTed the immunogen sequence against Cyclin A1, we did not see any significant sequence similarity. Thus, we do not predict ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"