Anti-Cyclin A2 antibody [E399] (ab32498)


  • Product nameAnti-Cyclin A2 antibody [E399]
    See all Cyclin A2 primary antibodies
  • Description
    Rabbit monoclonal [E399] to Cyclin A2
  • Specificityab32498 recognises Cyclin A2.
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cyclin A2 aa 100-200 (N terminal).

  • Epitopeab32498 reacts with an epitope located in the N terminal region of Cyclin A2
  • Positive control
    • WB: HeLa, K562 and Jurkat cell lysate. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab32498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 48 kDa.
ICC/IF 1/250 - 1/500.
IP 1/60.
  • Application notesIs unsuitable for Flow Cyt or IHC.
  • Target

    • FunctionEssential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCN1 antibody
      • CCNA antibody
      • Ccna2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Anti-Cyclin A2 antibody [E399] images

    • All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate
      Lane 3 : Jurkat whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

      The lower band is unidentified.

    • Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.

    References for Anti-Cyclin A2 antibody [E399] (ab32498)

    This product has been referenced in:
    • Wei X  et al. Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma. Cancer Lett 351:117-25 (2014). WB ; Human . Read more (PubMed: 24846301) »
    • Yajima H  et al. The complexity of DNA double strand breaks is a critical factor enhancing end-resection. DNA Repair (Amst) 12:936-46 (2013). Read more (PubMed: 24041488) »

    See all 3 Publications for this product

    Product Wall

    Ich denke wirklich, dass Ihr Problem mit diesen Aks nicht auf den WB begrenzt ist , da mir die ICC Färbung nicht spezifisch erscheint. Ich würde die Färbung definitiv eher nuklear lokalisiert erwarten, und das ist sie einfach nicht.
    Wir verwenden ...

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    Ich habe Ihnen einen Blot mit dem ab32498 an diese Email gehängt: von einem Schmier keine Spur! Was mir allerdings aufgefallen ist, ist dass der Verdünnungsfaktor um 10 verkleinert wurde. Ich habe unser Datenblatt dementsprechend verändert. Falls ...

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    Thank you for your inquiry. The lab has not tested this antibody for its cross-reactivity with Cyclin A1. But when we BLASTed the immunogen sequence against Cyclin A1, we did not see any significant sequence similarity. Thus, we do not predict ...

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