Recombinant
RabMAb

Anti-Cyclin A2 antibody [E399] (ab32498)

Overview

  • Product name
    Anti-Cyclin A2 antibody [E399]
    See all Cyclin A2 primary antibodies
  • Description
    Rabbit monoclonal [E399] to Cyclin A2
  • Specificity
    ab32498 recognises Cyclin A2.
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cyclin A2 aa 100-200 (N terminal).

  • Epitope
    ab32498 reacts with an epitope located in the N terminal region of Cyclin A2
  • Positive control
    • WB: HeLa, K562 and Jurkat cell lysate. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 48 kDa.
ICC/IF 1/250 - 1/500.
IP 1/60.
  • Application notes
    Is unsuitable for Flow Cyt or IHC.
  • Target

    • Function
      Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • Sequence similarities
      Belongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stage
      Accumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Cellular localization
      Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCN1 antibody
      • CCNA antibody
      • Ccna2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Images

    • All lanes : Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate
      Lane 3 : Jurkat whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

      The lower band is unidentified.

    • Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.

    References

    This product has been referenced in:
    • Wei X  et al. Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma. Cancer Lett 351:117-25 (2014). WB ; Human . Read more (PubMed: 24846301) »
    • Wu Z  et al. The inhibitory role of Mir-29 in growth of breast cancer cells. J Exp Clin Cancer Res 32:98 (2013). WB . Read more (PubMed: 24289849) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Ich denke wirklich, dass Ihr Problem mit diesen Aks nicht auf den WB begrenzt ist , da mir die ICC Färbung nicht spezifisch erscheint. Ich würde die Färbung definitiv eher nuklear lokalisiert erwarten, und das ist sie einfach nicht.
    Wir verwenden ...

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    Ich habe Ihnen einen Blot mit dem ab32498 an diese Email gehängt: von einem Schmier keine Spur! Was mir allerdings aufgefallen ist, ist dass der Verdünnungsfaktor um 10 verkleinert wurde. Ich habe unser Datenblatt dementsprechend verändert. Falls ...

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    Thank you for your inquiry. The lab has not tested this antibody for its cross-reactivity with Cyclin A1. But when we BLASTed the immunogen sequence against Cyclin A1, we did not see any significant sequence similarity. Thus, we do not predict ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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