Anti-Cyclin B1 antibody [7A9] (ab49215)

Overview

  • Product nameAnti-Cyclin B1 antibody [7A9]See all Cyclin B1 primary antibodies ...
  • Description
    Mouse monoclonal [7A9] to Cyclin B1
  • Tested applicationsWB, Flow Cyt, IHC-P, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Human Tonsil tissue: for IHC Raji cell line: for WB HT-29/219 cell line: for Indirect flow cytometry

Properties

Applications

Our Abpromise guarantee covers the use of ab49215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/50 - 1/100. Predicted molecular weight: 48 kDa.
Flow Cyt Use at an assay dependent dilution. Indirect.
IHC-P Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cyclin B1 (ab2949). For sandwich ELISA, use this antibody as Capture at 0.2 µg/ml with Rabbit polyclonal to Cyclin B1 (ab2949) as Detection.
  • Application notesIs unsuitable for IHC-Fr.
  • Target

    • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Post-translational
      modifications
      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
    • Target information above from: UniProt accession P14635 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CCNB 1 antibody
      • CCNB antibody
      • CCNB1 antibody
      • CCNB1_HUMAN antibody
      • G2 mitotic specific cyclin B1 antibody
      • G2/mitotic-specific cyclin-B1 antibody
      see all

    Anti-Cyclin B1 antibody [7A9] images

    Protocols

    References for Anti-Cyclin B1 antibody [7A9] (ab49215)

    ab49215 has not yet been referenced specifically in any publications.

    Product Wall

    The immunogen was whole recombinant full length protein (Human) and we have not carried out epitope mapping. Hence, unfortunately, we are unable to provide any information about the possible epitope for this clone.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"