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Anti-Cyclin B1 antibody [7A9] (ab49215)

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Overview

  • Product nameAnti-Cyclin B1 antibody [7A9]See all Cyclin B1 primary antibodies ...
  • Description
    Mouse monoclonal [7A9] to Cyclin B1
  • Tested applicationsWB, Flow Cyt, IHC-P, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive controlHuman Tonsil tissue: for IHC Raji cell line: for WB HT-29/219 cell line: for Indirect flow cytometry

Properties

Applications

Our Abpromise guarantee covers the use of ab49215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/50 - 1/100. Predicted molecular weight: 48 kDa.
Flow Cyt Flow Cyt: Use at an assay dependent dilution. Indirect.
IHC-P IHC-P: Use at an assay dependent dilution.
Sandwich ELISA sELISA: Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cyclin B1 (ab2949). For sandwich ELISA, use this antibody as Capture at 0.2 µg/ml with Rabbit polyclonal to Cyclin B1 (ab2949) as Detection.
  • Application notesIs unsuitable for or IHC-Fr.

    • Target

    • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Post-translational
      modifications      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

      • Target information above from: UniProt accession P14635 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CCNB 1 antibodyCCNB antibodyCCNB1 antibody
          CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody
        see all

      Anti-Cyclin B1 antibody [7A9] images

      Protocols

      References for Anti-Cyclin B1 antibody [7A9] (ab49215)

      ab49215 has not yet been referenced specifically in any publications.

      Publishing research using ab49215 ? Please let us know so that we can cite the reference in this datasheet

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      we bought the product "Cyclin B1 antibody [7A9] (ab49215)" from your company and we are very satisfied with it. Currently we are using a truncated version of Cyclin B1 and I would like to ask which part of the protein is recognized by this antibody, since...

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      The immunogen was whole recombinant full length protein (Human) and we have not carried out epitope mapping. Hence, unfortunately, we are unable to provide any information about the possible epitope for this clone.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"