Anti-Cyclin B1 antibody - BSA and Azide free (ab80793)
Overview
- Product nameAnti-Cyclin B1 antibody - BSA and Azide freeSee all Cyclin B1 primary antibodies ...
- DescriptionRabbit polyclonal to Cyclin B1 - BSA and Azide free
- Tested applicationsWB, IP, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A synthetic peptide derived from the C-terminal of Human Cyclin B1.
- EpitopeC-terminal
- Positive controlJurkat, Ls174T or HeLa cells. Breast carcinoma.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 10mM PBS, pH 7.4 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab80793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IP | |
| IHC-P |
IP: Use at 10µg/mg of lysate. Native and Denatured.
WB: Use at an assay dependent dilution. Predicted molecular weight: 48 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
- Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
- Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
- Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. - Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
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Database links
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Entrez Gene: 25203 Rat
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- SwissProt: P30277 Rat
- Unigene: 23960 Human
- Unigene: 260114 Mouse
- Unigene: 380027 Mouse
- Unigene: 482545 Mouse
- Unigene: 9232 Rat
see all
Target information above from: UniProt accession
P14635
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CCNB 1 antibodyCCNB antibodyCCNB1 antibody
- CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody
see all
Anti-Cyclin B1 antibody - BSA and Azide free images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin B1 antibody - BSA and Azide free (ab80793)Formalin-fixed, paraffin-embedded human breast cancer stained with ab80793 at 20µg/ml using a peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
References for Anti-Cyclin B1 antibody - BSA and Azide free (ab80793)
ab80793 has not yet been referenced specifically in any publications.
