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Anti-Cyclin B1 antibody [V152] (ab72)

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Overview

Product name

Anti-Cyclin B1 antibody [V152]
See all Cyclin B1 products (24) ...

Description

Mouse monoclonal [V152] to Cyclin B1

Specificity

Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression.

Tested applications

SDS-PAGE, ICC/IF, IHC (PFA fixed), Flow Cyt, WB, IHC-FoFr, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

His-tagged Hamster Cyclin B1 expressed in bacteria, harvested from inclusion bodies, extracted with 6M guanidine HCl and purified on Nickel beads.

Positive control

In Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; Daudi; K562; Jurkat; HEK293. In IHC, positive staining was observed in a Human Normal Tonsil formalin fixed paraffin embedded tissue section.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

V152

Myeloma

Sp2

Isotype

IgG1

Light chain type

unknown

Research areas

Cancer >> Cell cycle >> Cyclins >> Cyclin B family
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin B Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin B Family
Cell Biology >> Cell Cycle >> Cyclins

  • Western blot - Anti-Cyclin B1 antibody [V152] (ab72)Western blot - Anti-Cyclin B1 antibody [V152] (ab72) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [V152] (ab72)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [V152] (ab72) image (enlarge)

  • Western blot - Cyclin B1 antibody [V152] (ab72)Western blot - Cyclin B1 antibody [V152] (ab72) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab72 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or IP.

Target

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Developmental stage

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

Post-translational
modifications

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.

Cellular localization

Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accessionP14635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CCNB 1 antibody
  • CCNB antibody
  • ccnb1 antibody
  • CCNB1_HUMAN antibody
  • G2 mitotic specific cyclin B1 antibody
  • G2/mitotic-specific cyclin-B1 antibody
see all

Database links

see all

Anti-Cyclin B1 antibody [V152] images:

  Western blot - Anti-Cyclin B1 antibody [V152] (ab72)

Western blot - Anti-Cyclin B1 antibody [V152] (ab72)

All lanes : Anti-Cyclin B1 antibody [V152] (ab72) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3951)
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 48 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa,77 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 20 minutes

Abcam recommends using milk as the blocking agent.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [V152] (ab72)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [V152] (ab72)

IHC image of ab72 staining in Human Normal Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  Western blot - Cyclin B1 antibody [V152] (ab72)

Western blot - Cyclin B1 antibody [V152] (ab72)



Predicted band size : 48 kDa


Lanes 1-9 shows the Cyclin B1 wave as  HeLa cells enter and exit mitosis. HeLa cells were synchronised in the early S phase by double thymidine block then released to synchronously enter mitosis. Time points were taken at the points indicated.

HeLa whole cell lysate loaded at 25ug per lane. Ab72 used at 1/500 in reducing conditions in conjunction with goat anti mouse (HRP) 1/20000.

This image is courtesy of an Abreview submitted on 12 September 2005. We do not have any further information relating to this image.

See Abreview

References for Anti-Cyclin B1 antibody [V152] (ab72)

This product has been referenced in:

  • Holt JE  et al. The APC/C activator FZR1 coordinates the timing of meiotic resumption during prophase I arrest in mammalian oocytes. Development 138:905-13 (2011).Read more (PubMed: 21270054) »
  • Bernhardt ML  et al. Zinc requirement during meiosis I-meiosis II transition in mouse oocytes is independent of the MOS-MAPK pathway. Biol Reprod 84:526-36 (2011). WB.Read more (PubMed: 21076080) »

See all 21 publications for this product

Publishing research using ab72? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"