Anti-Cyclin B1 antibody [V92.1] (ab18221)

Overview

• Product nameAnti-Cyclin B1 antibody [V92.1]See all Cyclin B1 primary antibodies ...
• Description
  Mouse monoclonal [V92.1] to Cyclin B1
• SpecificityV92.1 can immunoprecipitate active CDK1/cyclin B1 complexes.
• Tested applicationsICC/IF, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Full length protein (Hamster).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberV92.1
• IsotypeIgG1
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin B family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin B Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin B Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Post-translational
  modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
• Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accession P14635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 891 Human
  • Entrez Gene: 268697 Mouse
  • Omim: 123836 Human
  • SwissProt: P14635 Human
  • SwissProt: P24860 Mouse
  • Unigene: 23960 Human
  • Unigene: 260114 Mouse
  • Unigene: 380027 Mouse

  • Unigene: 482545 Mouse

  see all

• Alternative names
  CCNB 1 antibodyCCNB antibodyCCNB1 antibody

  CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody

  see all

Anti-Cyclin B1 antibody [V92.1] images

• 

  Immunocytochemistry/ Immunofluorescence - Cyclin B1 antibody [V92.1] (ab18221)
  ICC/IF image of ab18221 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18221, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.