Overview

  • Product nameAnti-Cyclin B1 antibody [Y106]See all Cyclin B1 primary antibodies ...
  • Description
    Rabbit monoclonal [Y106] to Cyclin B1
  • SpecificityThis antibody is specific for Human cyclin B1. It does not cross-react with other cyclin family members.
  • Tested applicationsWB, IHC-P, Flow Cyt, ICC, IPmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human cyclin B1.

  • Epitopeab32053 reacts with an epitope located in teh C terminal region of Cyclin B1.
  • Positive control
    • Jurkat and Hela cell lysate.
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    This product is available conjugated to DyLight® 488 see ab139858.

    A trial size is available for this product.

Properties

Applications

Our Abpromise guarantee covers the use of ab32053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000 - 1/20000. Predicted molecular weight: 58 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/20.
ICC 1/50.
IP 1/100.

Target

  • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNB 1 antibody
    • CCNB antibody
    • CCNB1 antibody
    • CCNB1_HUMAN antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Anti-Cyclin B1 antibody [Y106] images

  • All lanes : Anti-Cyclin B1 antibody [Y106] (ab32053) at 1/20000 dilution

    Lane 1 : Hela cell lysate.
    Lane 2 : Jurkat cell lysate.


    Predicted band size : 58 kDa
  • ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
  • Overlay histogram showing Jurkat cells stained with ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32053, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Cyclin B1 antibody [Y106] (ab32053)

This product has been referenced in:
  • Wang H  et al. Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chin Med 9:15 (2014). WB ; Human . Read more (PubMed: 24872842) »
  • Khan S  et al. miR-379 regulates cyclin B1 expression and is decreased in breast cancer. PLoS One 8:e68753 (2013). Read more (PubMed: 23874748) »

See all 10 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (MCF7 cells)
Specification MCF7 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 11 2013

Thank you for contacting us.

I can confirm that the abs have IgG1 isotype.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Treatment double thymidine synchronization
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"