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Anti-Cyclin B1 antibody [Y106] (ab32053)

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Overview

Product name

Anti-Cyclin B1 antibody [Y106]
See all Cyclin B1 products (24) ...

Description

Rabbit monoclonal [Y106] to Cyclin B1

Specificity

This antibody is specific for Human cyclin B1. It does not cross-react with other cyclin family members.

Tested applications

WB, IHC-P, Flow Cyt, ICC, IPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to residues in the C-terminus of Human cyclin B1.

Epitope

ab32053 reacts with an epitope located in teh C terminal region of Cyclin B1.

Positive control

Jurkat and Hela cell lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

Y106

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Cyclins >> Cyclin B family
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin B Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin B Family
Cell Biology >> Cell Cycle >> Cyclins

  • Western blot - Cyclin B1 antibody [Y106] (ab32053)Western blot - Cyclin B1 antibody [Y106] (ab32053) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 antibody [Y106] (ab32053)Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 antibody [Y106] (ab32053) image (enlarge)

  • Flow Cytometry-Cyclin B1 antibody [Y106](ab32053)Flow Cytometry-Cyclin B1 antibody [Y106](ab32053) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Developmental stage

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

Post-translational
modifications

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.

Cellular localization

Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accessionP14635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CCNB 1 antibody
  • CCNB antibody
  • CCNB1 antibody
  • CCNB1_HUMAN antibody
  • G2 mitotic specific cyclin B1 antibody
  • G2/mitotic-specific cyclin-B1 antibody
see all

Database links

Anti-Cyclin B1 antibody [Y106] images:

  Western blot - Cyclin B1 antibody [Y106] (ab32053)

Western blot - Cyclin B1 antibody [Y106] (ab32053)

All lanes : Anti-Cyclin B1 antibody [Y106] (ab32053) at 1/20000 dilution

Lane 1 : Hela cell lysate.
Lane 2 : Jurkat cell lysate.


Predicted band size : 58 kDa

  Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 antibody [Y106] (ab32053)

Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 antibody [Y106] (ab32053)

ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

  Flow Cytometry-Cyclin B1 antibody [Y106](ab32053)

Flow Cytometry-Cyclin B1 antibody [Y106](ab32053)

Overlay histogram showing Jurkat cells stained with ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32053, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Cyclin B1 antibody [Y106] (ab32053)

This product has been referenced in:

  • Moisan F  et al. Deciphering the role of the ERCC2 gene polymorphism on anticancer drug sensitivity. Carcinogenesis 33:962-8 (2012). WB.Read more (PubMed: 22345163) »

See 1 publication for this product

Publishing research using ab32053? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"