Overview

  • Product nameAnti-Cyclin B1 antibody [Y106]
    See all Cyclin B1 primary antibodies
  • Description
    Rabbit monoclonal [Y106] to Cyclin B1
  • SpecificityThis antibody is specific for Human cyclin B1. It does not cross-react with other cyclin family members.
  • Tested applicationsWB, IHC-P, Flow Cyt, ICC, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human cyclin B1.

  • Epitopeab32053 reacts with an epitope located in teh C terminal region of Cyclin B1.
  • Positive control
    • Jurkat and Hela cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This product is available conjugated to DyLight® 488 see ab139858.

    A 40 µl trial size is available to purchase for this product.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000 - 1/20000. Predicted molecular weight: 58 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/20.
ICC 1/50.
IP 1/100.

Target

  • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNB 1 antibody
    • CCNB antibody
    • CCNB1 antibody
    • CCNB1_HUMAN antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Anti-Cyclin B1 antibody [Y106] images

  • All lanes : Anti-Cyclin B1 antibody [Y106] (ab32053) at 1/20000 dilution

    Lane 1 : Hela cell lysate.
    Lane 2 : Jurkat cell lysate.


    Predicted band size : 58 kDa
  • ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
  • Overlay histogram showing Jurkat cells stained with ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32053, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Cyclin B1 antibody [Y106] (ab32053)

This product has been referenced in:
  • Wang H  et al. Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chin Med 9:15 (2014). WB ; Human . Read more (PubMed: 24872842) »
  • Khan S  et al. miR-379 regulates cyclin B1 expression and is decreased in breast cancer. PLoS One 8:e68753 (2013). Read more (PubMed: 23874748) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH 8.6, 20 min 100C
Sample Human Tissue sections (tonsil)
Specification tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (MCF7 cells)
Specification MCF7 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 11 2013

Thank you for contacting us.

I can confirm that the abs have IgG1 isotype.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Treatment double thymidine synchronization
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"