Validated using a knockout cell line

Anti-Cyclin B1 antibody [Y106] (ab32053)


  • Product name
    Anti-Cyclin B1 antibody [Y106]
    See all Cyclin B1 primary antibodies
  • Description
    Rabbit monoclonal [Y106] to Cyclin B1
  • Specificity
    This antibody is specific for Human cyclin B1. It does not cross-react with other cyclin family members.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human Cyclin B1 aa 400-500 (C terminal).

  • Epitope
    ab32053 reacts with an epitope located in teh C terminal region of Cyclin B1.
  • Positive control
    • Jurkat and Hela cell lysate.
  • General notes

    A trial size is available to purchase for this product.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Cyclin B1 antibody (HRP) [Y106] (ab193977)

    Anti-Cyclin B1 antibody (Phycoerythrin) [Y106] (ab210615)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

For unpurified use at 1/50.

WB 1/50000. Predicted molecular weight: 58 kDa.

For unpurified use at 1/3000 - 1/20000.

IHC-P 1/250.
Flow Cyt 1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/20.

IP 1/20.

For unpurified use at 1/100.


  • Function
    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage
    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all


  • Predicted band size : 58 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: CCNB1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab32053 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Unpurified ab32053 was shown to specifically react with CCNB1 in wild-type HAP1 cells. No band was observed when CCNB1 knockout samples were examined. Wild-type and CCNB1 knockout samples were subjected to SDS-PAGE. Ab32053 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/3000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Unpurified ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

  • Unpurified ab32053 staining Cyclin B1 in the U2OS cell line from human by ICC/IF (Immunocytochemistry/immunofluorescence).Cells were fixed with formaldehyde,permeabilized with 1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 37°C.Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Overlay histogram showing Jurkat cells stained with unpurified ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32053, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • All lanes : Anti-Cyclin B1 antibody [Y106] (ab32053) at 1/20000 dilution (unpurified)

    Lane 1 : Hela cell lysate.
    Lane 2 : Jurkat cell lysate.

    Predicted band size : 58 kDa


This product has been referenced in:
  • Yang T  et al. Anti-tumor Efficiency of Lipid-coated Cisplatin Nanoparticles Co-loaded with MicroRNA-375. Theranostics 6:142-54 (2016). WB ; Human . Read more (PubMed: 26722380) »
  • Tseng YH  et al. Thyroid hormone suppresses expression of stathmin and associated tumor growth in hepatocellular carcinoma. Sci Rep 6:38756 (2016). Human . Read more (PubMed: 27934948) »

See all 26 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (U2OS)
Yes - 0.1% TritonX-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Stephanie Hills

Verified customer

Submitted Apr 27 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH 8.6, 20 min 100C
Human Tissue sections (tonsil)

Abcam user community

Verified customer

Submitted Aug 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Human Cell lysate - whole cell (MCF7 cells)
MCF7 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 11 2013

Thank you for contacting us.

I can confirm that the abs have IgG1 isotype.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
HeLa cells
double thymidine synchronization
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

Submitted May 14 2009


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