Anti-Cyclin B1 antibody [Y106] (ab32053)

Overview

• Product nameAnti-Cyclin B1 antibody [Y106]See all Cyclin B1 primary antibodies ...
• Description
  Rabbit monoclonal [Y106] to Cyclin B1
• SpecificityThis antibody is specific for Human cyclin B1. It does not cross-react with other cyclin family members.
• Tested applicationsWB, IHC-P, Flow Cyt, ICC, IP more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to residues in the C-terminus of Human cyclin B1.

• Epitopeab32053 reacts with an epitope located in teh C terminal region of Cyclin B1.
• Positive controlJurkat and Hela cell lysate.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberY106
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin B family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin B Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin B Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Post-translational
  modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
• Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accession P14635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 891 Human
  • Omim: 123836 Human
  • SwissProt: P14635 Human
  • Unigene: 23960 Human

• Alternative names
  CCNB 1 antibodyCCNB antibodyCCNB1 antibody

  CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody

  see all

Anti-Cyclin B1 antibody [Y106] images

• 

  Western blot - Cyclin B1 antibody [Y106] (ab32053)
  All lanes : Anti-Cyclin B1 antibody [Y106] (ab32053) at 1/20000 dilution
  
  Lane 1 : Hela cell lysate.
  Lane 2 : Jurkat cell lysate.
  
  
  Predicted band size : 58 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 antibody [Y106] (ab32053)
  ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
• 

  Flow Cytometry-Cyclin B1 antibody [Y106](ab32053)
  Overlay histogram showing Jurkat cells stained with ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32053, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.